Abiko H, Mizoi K, Suzuki J, Oba M, Yoshimoto T
Division of Neurosurgery, Tohoku University School of Medicine, Sendai, Japan.
No To Shinkei. 1987 Sep;39(9):847-54.
Recently there is the hypothesis proposing that ischemic brain damage is associated with intracellular accumulation of calcium (Ca++). Therefore a variety of experiments have been carried out to investigate whether a Ca++-entry blocker was able to protect against brain damage caused by ischemia. The purpose of the present experiment is to study the protective effects of a Ca++ antagonist, flunarizine, on cerebral ischemia. In this experiment fifteen dogs were subjected to ischemia, using the "canine model of the completely ischemic brain regulated with a perfusion method" in which the cerebral blood flow (CBF) can be fully regulated. Five animals served as untreated controls, ten received treatment with flunarizine (1 mg/kg in five dogs and 3 mg/kg in five dogs, respectively). This agent was administered intravenously 20 minutes prior to the production of ischemia, when cerebral blood flow was reduced to one-tenth its normal value while monitoring CBF by means of a laser-Doppler flow meter. After one hour CBF was restored and the recovery of electrical activity of the brain and the degree of brain swelling were observed for three hours. At the end of the experiments, the degree of extravasation of Evans blue in the excised brain was examined. With regard to the recovery of EEG, no recovery of EEG was seen subsequent to recirculation except one dog in the control group. Whereas in the groups treated with flunarizine, remarkable recovery of EEG was found following recirculation in a dose dependent fasion.(ABSTRACT TRUNCATED AT 250 WORDS)
最近有假说提出，缺血性脑损伤与细胞内钙（Ca++）蓄积有关。因此，已开展了各种实验来研究Ca++进入阻滞剂是否能够预防缺血所致的脑损伤。本实验的目的是研究Ca++拮抗剂氟桂利嗪对脑缺血的保护作用。在本实验中，对15只犬进行缺血处理，采用“通过灌注方法调节的完全缺血性脑犬模型”，在此模型中脑血流量（CBF）可得到充分调节。5只动物作为未处理对照，10只接受氟桂利嗪治疗（5只犬给予1mg/kg，5只犬给予3mg/kg）。在产生缺血前20分钟静脉注射该药物，此时通过激光多普勒血流仪监测脑血流量，使其降至正常值的十分之一。1小时后恢复脑血流量，并观察3小时脑电活动的恢复情况及脑肿胀程度。实验结束时，检查切除脑内伊文思蓝的外渗程度。关于脑电图的恢复，再灌注后除对照组1只犬外未见脑电图恢复。而在氟桂利嗪治疗组，再灌注后脑电图有明显的剂量依赖性恢复。（摘要截断于250字）
