Plaza Andrea, Rodríguez Lyanne, Concha-Meyer Anibal A, Cabezas René, Zurob Elsie, Merlet Gastón, Palomo Iván, Fuentes Eduardo
Centro de Estudios en Alimentos Procesados-CEAP, Conicyt, Programa Regional R19A10001, Gore Maule, Talca 3480094, Chile.
Thrombosis Research Center, Medical Technology School, Department of Clinical Biochemistry and Immunohematology, Faculty of Health Sciences, Universidad de Talca, Talca 3480094, Chile.
Plants (Basel). 2023 Mar 6;12(5):1188. doi: 10.3390/plants12051188.
Aqueous and ethanolic extracts of tomato pomace were examined with the aim of optimizing the extraction process of compounds with cardioprotective activity. Once the results of the ORAC response variables, total polyphenols, °Brix, and antiplatelet activity of the extracts were obtained, a multivariate statistical analysis was performed using the Statgraphics Centurion XIX software. This analysis showed that the most relevant positive effects in the inhibition of platelet aggregation were 83 ± 2% when using the agonist TRAP-6, when the working conditions were the type of tomato pomace conditioning (drum-drying process at 115 °C), phase ratio (1/8), type of solvent (ethanol 20%), and type of extraction (ultrasound-assisted solid-liquid extraction). The extracts with the best results were microencapsulated and characterized by HPLC. The presence of chlorogenic acid (0.729 mg/mg of dry sample) was found, a compound that has a potential cardioprotective effect documented in various studies, in addition to rutin (2.747 mg/mg of dry sample) and quercetin (0.255 mg/mg of dry sample). These results show that the extraction efficiency of compounds with cardioprotective activity depends largely on the polarity of the solvent, thus playing an important role in the antioxidant capacity of the extracts of tomato pomace.
对番茄渣的水提取物和乙醇提取物进行了研究，目的是优化具有心脏保护活性的化合物的提取工艺。一旦获得提取物的氧自由基吸收能力（ORAC）响应变量、总多酚、糖度和抗血小板活性的结果，便使用Statgraphics Centurion XIX软件进行多元统计分析。该分析表明，当使用激动剂TRAP-6时，在抑制血小板聚集方面最相关的积极效果为83±2%，此时的工作条件为番茄渣的处理类型（115℃鼓式干燥工艺）、相比（1/8）、溶剂类型（20%乙醇）和提取类型（超声辅助固液萃取）。将效果最佳的提取物进行微囊化处理，并通过高效液相色谱法（HPLC）进行表征。结果发现存在绿原酸（0.729毫克/毫克干样品），除芦丁（2.747毫克/毫克干样品）和槲皮素（0.255毫克/毫克干样品）外，该化合物在多项研究中已证明具有潜在的心脏保护作用。这些结果表明，具有心脏保护活性的化合物的提取效率在很大程度上取决于溶剂的极性，因此在番茄渣提取物的抗氧化能力中发挥着重要作用。
