通过单细胞 RNA 测序方法对树突状细胞造血的发育轨迹进行表征。

Characterization of Developmental Trajectories of Dendritic Cell Hematopoiesis Through Single-Cell RNA Sequencing Methods.

机构信息

Institute of Systems Immunology, Hamburg Center for Translational Immunology (HCTI), University Medical Center Hamburg-Eppendorf, Hamburg, Germany.

Singapore Immunology Network (SIgN), Agency for Science, Technology and Research, Singapore, Singapore.

出版信息

Methods Mol Biol. 2023;2618:375-385. doi: 10.1007/978-1-0716-2938-3_23.

DOI:10.1007/978-1-0716-2938-3_23

PMID:36905527

Abstract

Single-cell technologies have become valuable tools to trace dendritic cell differentiation trajectories. Here, we illustrate the workflow used for processing of mouse bone marrow for single-cell RNA sequencing and trajectory analyses, as done in Dress et al. (Nat Immunol 20:852-864, 2019). This short methodology is presented as a starting point for researchers just beginning to dive into the complex field of dendritic cell ontogeny and cellular development trajectory analyses.

摘要

单细胞技术已成为追踪树突状细胞分化轨迹的有用工具。在这里，我们展示了 Dress 等人在《自然免疫学》（Nat Immunol 20:852-864, 2019）中所使用的处理小鼠骨髓用于单细胞 RNA 测序和轨迹分析的工作流程。本简短方法旨在为刚刚开始涉足树突状细胞发生和细胞发育轨迹分析这一复杂领域的研究人员提供一个起点。