South Pratacultural Center, South China Agricultural University, Guangzhou 510642, China.
School of Biological Sciences and Technology, Liupanshui Normal University, Liupanshui 553004, China.
FEMS Microbiol Ecol. 2023 Mar 23;99(4). doi: 10.1093/femsec/fiad024.
Currently, our understanding of the effects of glaucousness on the population sizes of microbial communities on leaf surfaces is limited. The objective of this study was to investigate the influence of glaucousness and chemical composition on the colony-forming unit (CFU) counts of microbes on leaf surfaces. Various leaf morphological and physicochemical features, microbial CFU counts and glaucousness on the leaf surfaces of wheat (Triticum aestivum var. Shimai No.1), rye (Secale cereale var. Gannong No.1) and triticale (Triticale wittmack var. Gannong No.2) were assessed. The results showed that larger CFU counts of lactic acid bacteria (LAB) and yeasts were found on the non-glaucous leaf surfaces in wheat than on the glaucous leaf surfaces in rye and triticale. The CFU counts of LAB and yeasts were negatively correlated with the amount of soluble wax (P < 0.05), and positively correlated with the contents of starch and fructose in leaf tissue (P < 0.05), soluble and reduced sugars, soluble protein and free amino acids on leaf surfaces (P < 0.05). The CFU counts of microbes on leaf surfaces were mainly influenced by the level of available carbon sources and glaucousness of leaf surfaces.
目前，我们对叶片表面微生物群落的白变现象对其种群大小的影响的了解有限。本研究旨在调查白变和化学成分对叶片表面微生物菌落形成单位（CFU）计数的影响。对小麦（Triticum aestivum var. Shimai No.1）、黑麦（Secale cereale var. Gannong No.1）和小黑麦（Triticale wittmack var. Gannong No.2）叶片的各种叶片形态和理化特征、微生物 CFU 计数和叶片表面的白变进行了评估。结果表明，与黑麦和小黑麦的叶片相比，小麦的非白变叶片表面的乳酸菌（LAB）和酵母的 CFU 计数较大。LAB 和酵母的 CFU 计数与可溶蜡的含量呈负相关（P < 0.05），与叶片组织中的淀粉和果糖含量呈正相关（P < 0.05），与叶片表面的可溶和还原糖、可溶蛋白和游离氨基酸含量呈正相关（P < 0.05）。叶片表面微生物 CFU 计数主要受叶片表面可利用碳源水平和白变的影响。
