Dermatology and Venereology Department, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt.
Biochemistry Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
Arch Dermatol Res. 2023 Sep;315(7):2065-2071. doi: 10.1007/s00403-023-02586-6. Epub 2023 Mar 15.
Damage-associated molecular patterns (DAMPs) play a role in the pathogenesis of vitiligo. It has been established that the cold-inducible RNA-binding protein (CIRP), a member of the family of cold-shock proteins that respond to stress, is a DAMP molecule that promotes inflammation. The objective was to evaluate the serum and tissue CIRP expression in non-segmental vitiligo (NSV) patients. A sample of 40 participants, 20 NSV patients and 20 control groups of age- and sex-matched healthy individuals were included in this case-control study where the enzyme-linked immunosorbent assay was used in detecting the serum and tissue CIRP levels in participants. The serum and tissue CIRP levels significantly increased in NSV patients compared with the healthy controls, (165.35 ± 24.42, 226.29 ± 24.00 versus 59.81 ± 12.10, 105.86 ± 11.27 pg/ml, respectively) (P < 0.01). Serum and tissue CIRP are significantly correlated with each other (r = 0.641, P = 0.002). Except for a statistically significant positive correlation between CIRP tissue level and VASI (r = 0.539, P = 0.014), the CIRP Serum and tissue did not show any statistically significant correlations with different clinical parameters in patients. ROC curve shows that the cut-off point for serum and tissue CIRP level to differentiate between patients and controls was 86.5, 124.3 pg/ml, respectively, with 100.0% sensitivity, 100.0% specificity and 1.000 AUC for each of them. It is concluded that CIRP may have a crucial role in the pathogenesis of NSV and could be used as a marker for vitiligo and its extent with the need for further large-scale study.
损伤相关分子模式（DAMPs）在白癜风的发病机制中起作用。已经确定冷诱导 RNA 结合蛋白（CIRP）是冷休克蛋白家族的成员之一，该蛋白对压力作出反应，是一种促进炎症的 DAM 分子。目的是评估非节段性白癜风（NSV）患者的血清和组织 CIRP 表达。在这项病例对照研究中，纳入了 40 名参与者，其中 20 名 NSV 患者和 20 名年龄和性别匹配的健康对照组，使用酶联免疫吸附测定法检测参与者的血清和组织 CIRP 水平。与健康对照组相比，NSV 患者的血清和组织 CIRP 水平显著升高，（165.35±24.42，226.29±24.00 与 59.81±12.10，105.86±11.27 pg/ml，分别）（P<0.01）。血清和组织 CIRP 之间存在显著相关性（r=0.641，P=0.002）。除了 CIRP 组织水平与 VASI 之间存在统计学显著的正相关（r=0.539，P=0.014）外，CIRP 血清和组织与患者的不同临床参数之间没有显示出任何统计学显著相关性。ROC 曲线显示，血清和组织 CIRP 水平区分患者和对照组的截断点分别为 86.5、124.3 pg/ml，具有 100.0%的敏感性、100.0%的特异性和 1.000 AUC。结论：CIRP 可能在 NSV 的发病机制中起关键作用，可作为白癜风及其严重程度的标志物，需要进一步进行大规模研究。
