Shaker Olfat G, Abd Elrahim Talal A, Azzam Somia, El-Zook Mai Mohamed, Aboraia Nesreen M
Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Department of Dermatology, Faculty of Medicine, Fayoum University, Fayoum, Egypt.
Sci Rep. 2025 Jan 6;15(1):1031. doi: 10.1038/s41598-024-83489-y.
Vitiligo is a common long-term depigmenting skin disorder that is characterized by patches of skin losing their pigment. To evaluate serum/tissue levels of miR-16, 146a, 19b and 720 in vitiligo patients and healthy controls, also analyzing the correlations between all biomarkers to indicate whether those can be used to early diagnose vitiligo patients. Forty-subjects were included, divided into two equal groups, 20 healthy matched individuals and 20 vitiligo patients. For all groups a 5 mL venous blood sample for serum isolation was taken and analyzed for the serum level of miRNAs. For tissue analysis, 3 mm biopsy was taken. For all patients Vitiligo area scoring index (VASI), vitiligo disease activity (VIDA), disease duration and extent percentages were calculated. No significant difference was observed in age and sex ratio among the two groups (p > 0.05). Serum, expression levels for miR-16, 146a, and 19b were overexpressed in vitiligo patients as compared to healthy controls with p-value 0.000 for all biomarkers. While miR-720 was reported low in vitiligo patients compared to controls (p = 0.000). For tissue samples, miR-16, 146a, 19b were overexpressed in vitiligo patients with p-values 0.000, 0.000 and < 0.001 respectively, while for the expression level of miR-720 in tissue, the level was low compared to controls (p = 0.000). There are positive correlations between VASI and miR-16, 146a in serum and miR-146a in tissue. Also positive correlations between disease extent and both miR-16 and miR-146a in serum and in tissue was found. A negative correlation between VIDA and miR-720 in serum was found. Various correlations between the selected miRNAs were reported. Based upon the expression levels of miR-16, 146a, 19b and 720 in both serum and tissue, these biomarkers can be used as early indicators for vitiligo.
白癜风是一种常见的慢性色素脱失性皮肤病，其特征是皮肤出现色素脱失斑。为了评估白癜风患者和健康对照者血清/组织中miR-16、146a、19b和720的水平，并分析所有生物标志物之间的相关性，以确定它们是否可用于早期诊断白癜风患者。研究纳入了40名受试者，分为两组，每组20人，一组为健康匹配个体，另一组为20名白癜风患者。对所有组均采集5 mL静脉血样用于分离血清，并分析血清中miRNA的水平。对于组织分析，采集3 mm活检组织。计算所有患者的白癜风面积评分指数（VASI）、白癜风疾病活动度（VIDA）、病程和病变范围百分比。两组之间在年龄和性别比例上未观察到显著差异（p＞0.05）。与健康对照相比，白癜风患者血清中miR-16、146a和19b的表达水平均上调，所有生物标志物的p值均为0.000。而与对照组相比，白癜风患者中miR-720的水平较低（p = 0.000）。对于组织样本，白癜风患者中miR-16、146a、19b的表达上调，p值分别为0.000、0.000和＜0.001，而组织中miR-720的表达水平与对照组相比则较低（p = 0.000）。血清中VASI与miR-16、146a以及组织中miR-146a之间存在正相关。血清和组织中病变范围与miR-16和miR-146a之间也存在正相关。发现血清中VIDA与miR-720之间存在负相关。还报道了所选miRNA之间的各种相关性。基于血清和组织中miR-16、146a、19b和720的表达水平，这些生物标志物可作为白癜风的早期指标。
