Adukwu Benjamin U, Nwosu Sebastian N N, Emele Felix E, Uba-Obiano Chizoba, Onyiaorah Adaora A
Guinness Eye Centre Onitsha, Nigeria.
Department of Ophthalmology, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria.
J West Afr Coll Surg. 2023 Jan-Mar;13(1):79-83. doi: 10.4103/jwas.jwas_216_22. Epub 2023 Jan 18.
The aim of this study is to determine the hospital incidence and pattern of conjunctival fungal flora in adult patients at the Guinness Eye Center Onitsha, Nigeria.
New adult patients, without anterior segment disease, were randomly recruited. Using a sterile swab stick, specimen was taken from the inferior conjunctival fornix of each participant's right eye and inoculated into Sabouraud dextrose agar slant in a test tube and incubated at 27°C. The specimens were examined for fungal growth every 48 h for 4 weeks. Specimens with fungal growth were further examined under a high power microscope for fungal identification and characterization.
A total of 225 patients (105 males, 120 females) were examined. The age range was 18-75 years; mean age was 41 ± 17.1 years; 62 (27.6%) were culture-positive: 25 (40.3%) were males and 37 (59.7%) were females ( >0.05). Both moulds and yeasts were isolated with moulds constituting 44 (74.2%). [26 (41.9%)] and [16 (25.8%)] were the commonest organisms. Participants >60 years had the greatest burden. Pensioners (61.5%), traders (44.0%), farmers (30.1%), and artisans (27.3%) were occupational groups with significantly higher proportions of culture-positive specimens ( < 0.05).
Over a quarter of new adult patients without anterior segment disease harbour fungi, some of which are pathogenic, in their conjunctival fungal organisms. While and were the commonest isolates, older participants, pensioners, traders, farmers, and artisans had significantly higher proportion of culture-positive specimens. These findings should be considered when formulating pre-operative guidelines for ocular surgery in our environment.
本研究旨在确定尼日利亚奥尼查吉尼斯眼科中心成年患者结膜真菌菌群的医院发病率及模式。
随机招募无前节疾病的成年新患者。使用无菌拭子棒，从每位参与者右眼的下结膜穹窿采集标本，接种于试管中的沙氏葡萄糖琼脂斜面上，在27°C下培养。每48小时检查标本是否有真菌生长，持续4周。对有真菌生长的标本在高倍显微镜下进一步检查以进行真菌鉴定和特征描述。
共检查了225例患者（男性105例，女性120例）。年龄范围为18 - 75岁；平均年龄为41±17.1岁；62例（27.6%）培养阳性：男性25例（40.3%），女性37例（59.7%）（P>0.05）。霉菌和酵母菌均有分离，其中霉菌占44例（74.2%）。烟曲霉[26例（41.9%）]和白色念珠菌[16例（25.8%）]是最常见的菌种。60岁以上的参与者负担最重。退休人员（61.5%）、商人（44.0%）、农民（30.1%）和工匠（27.3%）是培养阳性标本比例显著较高的职业群体（P<0.05）。
超过四分之一无前节疾病的成年新患者结膜真菌菌群中携带真菌，其中一些具有致病性。虽然烟曲霉和白色念珠菌是最常见的分离菌种，但年龄较大的参与者、退休人员、商人、农民和工匠的培养阳性标本比例显著较高。在制定我们环境下眼科手术的术前指南时应考虑这些发现。
