Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Porto, Portugal.
Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
Int J Antimicrob Agents. 2023 May;61(5):106788. doi: 10.1016/j.ijantimicag.2023.106788. Epub 2023 Mar 15.
Pseudomonas aeruginosa (P. aeruginosa) are ubiquitous opportunistic pathogens that combine intrinsic and acquired multidrug resistance phenotypes. Due to different types of acquired genes, carbapenem resistance has been expanding in this species. This study hypothesised that the spread of carbapenem resistance among P. aeruginosa is influenced by phylogenomic features, being distinct for different genes.
To test this hypothesis, the genomes of P. aeruginosa harbouring bla or bla genes were compared. The bla gene was selected because, although frequent, it is almost restricted to this species and bla gene due to its wide interspecies distribution. A group of genomes harbouring the genes bla (n = 116) or bla (n = 27), available in GenBank, was characterised based on core phylogenomic analysis, functional categories in the accessory genome and mobile genetic elements flanking the selected genes.
Most bla gene hosts belonged to multilocus sequence types (ST) ST111 (n = 32 of 116) and ST233 (n = 27 of 116) and were reported in Europe (n = 75 of 116). The bla gene hosts were distributed by different STs (ST38, ST773, ST235, ST357 and ST654), frequently from Asia (n = 11 of 27). Significant differences in the prevalence of functional protein/enzyme annotations per number of accessory genomes were observed between bla+ and bla+. The bla gene was frequently inserted in the Tn402-like and Tn21 transposons family and rarely in IS6100, while bla gene was preferentially flanked by ISAba125 and ble genes or associated with IS91 insertion sequence.
The hypothesis that carbapenem resistance gene acquisition is not random among phylogenomic lineages was confirmed, suggesting the importance of phylogeny in the dissemination of antibiotic resistance genes.
铜绿假单胞菌（P. aeruginosa）是一种普遍存在的机会致病菌，具有固有和获得的多药耐药表型。由于获得性基因的不同类型，该物种的碳青霉烯类耐药性一直在扩大。本研究假设，铜绿假单胞菌中碳青霉烯类耐药性的传播受到系统发育特征的影响，不同基因的传播方式不同。
为了验证这一假设，比较了携带 bla 或 bla 基因的铜绿假单胞菌基因组。选择 bla 基因是因为，尽管它很常见，但它几乎仅限于该物种，而 bla 基因由于其广泛的种间分布。从系统发育核心分析、辅助基因组中的功能类别以及选择基因侧翼的移动遗传元件等方面，对一组含有 bla 基因（n=116）或 bla 基因（n=27）的基因组进行了特征描述，这些基因组可在 GenBank 中获得。
大多数 bla 基因宿主属于多位点序列类型（ST）ST111（n=116 中的 32 个）和 ST233（n=116 中的 27 个），并在欧洲报道（n=116 中的 75 个）。bla 基因宿主分布在不同的 ST（ST38、ST773、ST235、ST357 和 ST654）中，主要来自亚洲（n=27 中的 11 个）。bla+和 bla+的辅助基因组中功能蛋白/酶注释的数量存在显著差异。bla 基因经常插入 Tn402 样和 Tn21 转座子家族，很少插入 IS6100，而 bla 基因则优先被 ISAba125 和 ble 基因侧翼包围，或与 IS91 插入序列相关。
碳青霉烯类耐药基因获得在系统发育谱系中并非随机的假设得到了证实，这表明了系统发育在抗生素耐药基因传播中的重要性。
