小鼠组织中基础和胰岛素刺激的葡萄糖摄取的体内测量方案。
Protocol for in vivo measurement of basal and insulin-stimulated glucose uptake in mouse tissues.
作者信息
Zhao Meng, Wat Lianna W, Svensson Katrin J
机构信息
Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA; Stanford Diabetes Research Center, Stanford University School of Medicine, Stanford, CA 94305, USA; Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA.
Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA; Stanford Diabetes Research Center, Stanford University School of Medicine, Stanford, CA 94305, USA; Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA.
出版信息
STAR Protoc. 2023 Mar 17;4(2):102179. doi: 10.1016/j.xpro.2023.102179.
Here, we present an in vivo protocol for measuring basal and insulin-stimulated glucose uptake in tissues from mice. We describe steps for administering 2-deoxy-D-[1,2-H]glucose in the presence or absence of insulin via intraperitoneal injections. We then detail tissue collection, tissue processing to measure H counts on a scintillation counter, and data interpretation. This protocol can be applied to other glucoregulatory hormones, genetic mouse models, and other species. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2021)..
在此，我们展示了一种用于测量小鼠组织中基础和胰岛素刺激的葡萄糖摄取的体内实验方案。我们描述了通过腹腔注射在有或没有胰岛素的情况下给予2-脱氧-D-[1,2-H]葡萄糖的步骤。然后，我们详细介绍了组织收集、组织处理以在闪烁计数器上测量H计数以及数据解释。该方案可应用于其他血糖调节激素、基因小鼠模型和其他物种。有关该方案使用和执行的完整详细信息，请参考Jiang等人（2021年）的研究。
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