Pramanik Rinka, Bodawar Narendra, Brahme Aashay, Kamble Sanjay, Dharne Mahesh
National Collection of Industrial Microorganisms (NCIM), Biochemical Sciences Division, CSIR, National Chemical Laboratory (NCL), Pune 411008, India.
Chemical Engineering and Process Development (CEPD) Division, CSIR, National Chemical Laboratory (NCL), Pune 411008, India.
J Environ Chem Eng. 2023 Jun;11(3):109673. doi: 10.1016/j.jece.2023.109673. Epub 2023 Mar 11.
Presence of SARS-CoV-2 in wastewater is a major concern as the wastewater meets rivers and other water bodies and is used by the population for various purposes. Hence it is very important to treat sewage water in an efficient manner in order to reduce the public health risk. In the present work, various advanced oxidation processes (AOPs) have been evaluated for disinfection of SARS-CoV-2 from sewage water collected from STP inlet of academic institutional residential. The sewage water was subjected to ten AOPs, which include Ozone (O), Hydrodynamic cavitation (HC), Ultraviolet radiation (UV), and their hybrid combinations like HC/O, HC/O/HO, HC/HO O/UV, UV/HO, UV/HO/O and O/HO to reduce SARS-CoV-2 viral load. Further, AOP treated sewage water was subjected to total nucleic acid isolation followed by RT-qPCR for viral load estimation. The sewage water treatment techniques were evaluated based on their viral concentration-reducing efficiency. It was found that ozone and ozone-coupled hybrid AOPs showed the most promising result with more than 98 % SARS-CoV-2 viral load reducing efficiency from sewage water. Interestingly, the best six AOPs used in this study significantly reduced both the SARS-CoV-2 and PMMoV (faecal indicator) viral load and improved water quality in terms of increasing DO and decreasing TOC.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）存在于废水中是一个重大问题，因为废水会汇入河流和其他水体，且被人们用于各种用途。因此，高效处理污水以降低公共卫生风险非常重要。在本研究中，对从学术机构住宅区污水处理厂进水口收集的污水中用于消毒SARS-CoV-2的各种高级氧化工艺（AOPs）进行了评估。该污水接受了十种AOPs处理，包括臭氧（O）、水力空化（HC）、紫外线辐射（UV）及其混合组合，如HC/O、HC/O/HO、HC/HO、O/UV、UV/HO、UV/HO/O和O/HO，以降低SARS-CoV-2病毒载量。此外，对经过AOP处理的污水进行总核酸分离，然后通过逆转录定量聚合酶链反应（RT-qPCR）来估计病毒载量。根据污水处理技术降低病毒浓度的效率对其进行了评估。结果发现，臭氧和臭氧耦合的混合AOPs显示出最有前景的结果，污水中SARS-CoV-2病毒载量降低效率超过98%。有趣的是，本研究中使用的最佳六种AOPs显著降低了SARS-CoV-2和人多瘤病毒（PMMoV，粪便指示物）的病毒载量，并在提高溶解氧（DO）和降低总有机碳（TOC）方面改善了水质。
