Song Siyuan, Jin Kai, Wang Shuai, Yang Ce, Zhou Jingxin, Chen Zhiqing, Ye Juan
Eye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
School of Mechanical, Electrical and Information Engineering, Shandong University, Weihai, China.
Front Cell Dev Biol. 2023 Mar 8;11:1157497. doi: 10.3389/fcell.2023.1157497. eCollection 2023.
To explain the biological role of cytokines in the eye and the possible role of cytokines in the pathogenesis of neovascular age-related macular degeneration (nAMD) by comparing the correlation between cytokine of aqueous humor concentration and optical coherence tomography (OCT) retinal fluid. Spectral-domain OCT (SD-OCT) images and aqueous humor samples were collected from 20 nAMD patient's three clinical visits. Retinal fluid volume in OCT was automatically quantified using deep learning--Deeplabv3+. Eighteen cytokines were detected in aqueous humor using the Luminex technology. OCT fluid volume measurements were correlated with changes in aqueous humor cytokine levels using Pearson's correlation coefficient (PCC). The patients with intraretinal fluid (IRF) showed significantly lower levels of cytokines, such as C-X-C motif chemokine ligand 2 (CXCL2) ( = 0.03) and CXCL11 ( = 0.009), compared with the patients without IRF. And the IRF volume was negatively correlated with CXCL2 ( = -0.407, = 0.048) and CXCL11 ( = -0.410, = 0.046) concentration in the patients with IRF. Meanwhile, the subretinal fluid (SRF) volume was positively correlated with vascular endothelial growth factor (VEGF) concentration (r = 0.299, = 0.027) and negatively correlated with interleukin (IL)-36β concentration ( = -0.295, = 0.029) in the patients with SRF. Decreased level of VEGF was associated with decreased OCT-based retinal fluid volume in nAMD patients, while increased levels of CXCL2, CXCL11, and IL-36β were associated with decreased OCT-based retinal fluid volume in nAMD patients, which may suggest a role for inflammatory cytokines in retinal morphological changes and pathogenesis of nAMD patients.
通过比较房水细胞因子浓度与光学相干断层扫描（OCT）视网膜液之间的相关性，来解释细胞因子在眼部的生物学作用以及细胞因子在新生血管性年龄相关性黄斑变性（nAMD）发病机制中的可能作用。从20例nAMD患者的三次临床就诊中收集了光谱域OCT（SD-OCT）图像和房水样本。使用深度学习——深度卷积神经网络v3+自动定量OCT中的视网膜液体积。采用Luminex技术检测房水中的18种细胞因子。使用Pearson相关系数（PCC）将OCT液体积测量值与房水细胞因子水平的变化相关联。与无视网膜内液（IRF）的患者相比，有IRF的患者细胞因子水平显著降低，如C-X-C基序趋化因子配体2（CXCL2）（P = 0.03）和CXCL11（P = 0.009）。在有IRF的患者中，IRF体积与CXCL2（r = -0.407，P = 0.048）和CXCL11（r = -0.410，P = 0.046）浓度呈负相关。同时，在有视网膜下液（SRF）的患者中，SRF体积与血管内皮生长因子（VEGF）浓度呈正相关（r = 0.299，P = 0.027），与白细胞介素（IL）-36β浓度呈负相关（r = -0.295，P = 0.029）。nAMD患者中VEGF水平降低与基于OCT的视网膜液体积减少相关，而CXCL2、CXCL11和IL-36β水平升高与nAMD患者中基于OCT的视网膜液体积减少相关，这可能提示炎性细胞因子在nAMD患者视网膜形态变化和发病机制中起作用。
