Department of Neurobiology, School of Biological Sciences, University of California San Diego, La Jolla, CA, USA.
Departament de Patologia i Terapèutica Experimental, Institut de Neurociències, Universitat de Barcelona, L'Hospitalet de Llobregat, Catalonia, Spain.
Nat Methods. 2023 May;20(5):682-685. doi: 10.1038/s41592-023-01819-w. Epub 2023 Mar 27.
Photoactivatable drugs and peptides can drive quantitative studies into receptor signaling with high spatiotemporal precision, yet few are compatible with behavioral studies in mammals. We developed CNV-Y-DAMGO-a caged derivative of the mu opioid receptor-selective peptide agonist DAMGO. Photoactivation in the mouse ventral tegmental area produced an opioid-dependent increase in locomotion within seconds of illumination. These results demonstrate the power of in vivo photopharmacology for dynamic studies into animal behavior.
光激活药物和肽可以在高时空精度下驱动受体信号的定量研究，但很少有药物与哺乳动物的行为研究兼容。我们开发了 CNV-Y-DAMGO，一种 µ 阿片受体选择性肽激动剂 DAMGO 的笼状衍生物。在小鼠腹侧被盖区进行光激活，在光照后的几秒钟内产生了阿片类药物依赖性的运动增加。这些结果证明了体内光药理学在动物行为的动态研究中的强大功能。
