Dégi János, Morariu Sorin, Simiz Florin, Herman Viorel, Beteg Florin, Dégi Diana Maria
Department of Infectious Diseases and Preventive Medicine, University of Life Sciences "King Mihai I" from Timișoara, 300645 Timisoara, Romania.
Department of Parasitology, Parasitic Diseases and Dermatology, University of Life Sciences "King Mihai I" from Timișoara, 300645 Timisoara, Romania.
Antibiotics (Basel). 2024 Dec 2;13(12):1162. doi: 10.3390/antibiotics13121162.
Antimicrobial resistance (AMR) has surfaced as a critical challenge to public health on a global scale. The precise and swift identification of resistance to antimicrobial agents, along with timely and suitable antimicrobial therapy paired with effective stewardship practices, is crucial for managing the rise and dissemination of antimicrobial resistance. The objective of our investigation was to outline the antimicrobial resistance profile of spp., a significant contributor to canine otitis, a prevalent condition in dogs, isolated in Western Romania.
All data were collected from clinical cases of canine otitis externa which presented at the University Clinic of the Faculty of Veterinary Medicine in Timișoara/Romania. A clinical evaluation was conducted, from which era swabs are usually collected and sent for analysis at the laboratory. Laboratory analysis included the microbiological examination for identifying spp. and determining antibiotic susceptibility phenotypes. Statistical analysis was implemented on all data that were collected. The ear swabs were processed with standard procedures for cultivating and identifying bacteria. The resulting subcultures were processed to determine the staphylococcal species on the GP ID Cards of the Vitek 2 automatic system. The antimicrobial susceptibility profiles were detected by the Vitek 2 system using an AST-GP80 card. These isolated spp. strains were further processed by real-time PCR and PCR-RFLP.
Of all the auricular exudate samples analyzed, 76 were positive for spp. (59.38%). Within these, in 82% of auricular samples, six distinct spp. were identified (, , , and ). Our data indicate that the PCR-RFLP assay is a practical approach to identification, allowing for discrimination from the other (SIG) species and important staphylococcal pathogens of dogs. The highest frequency of resistant isolates was detected against tetracycline (21/34; 61.76%; -value 0.003), gentamicin (20/34; 58.82%), and kanamycin (20/34; 58.82%).
These results are essential to guide the prudent use of antibiotics in veterinary medicine. They will also help design efficient control strategies and measure their effectiveness.
抗菌药物耐药性（AMR）已成为全球公共卫生领域的一项严峻挑战。准确、快速地识别对抗菌药物的耐药性，以及及时、适当的抗菌治疗并结合有效的管理措施，对于应对抗菌药物耐药性的增加和传播至关重要。我们调查的目的是概述在罗马尼亚西部分离出的犬中耳炎（犬类常见疾病）的主要病原体—— spp.的抗菌药物耐药情况。
所有数据均收集自蒂米什瓦拉/罗马尼亚兽医学院大学诊所的犬外耳炎临床病例。进行了临床评估，通常从中采集拭子并送往实验室进行分析。实验室分析包括微生物学检查，以鉴定 spp. 并确定抗生素敏感性表型。对收集到的所有数据进行了统计分析。耳拭子采用标准程序进行细菌培养和鉴定。将所得的传代培养物进行处理，以在Vitek 2自动系统的GP ID卡上确定葡萄球菌种类。使用AST-GP80卡通过Vitek 2系统检测抗菌药物敏感性谱。这些分离出的 spp. 菌株通过实时PCR和PCR-RFLP进一步处理。
在所有分析的耳分泌物样本中，76份 spp. 呈阳性（59.38%）。其中，在82%的耳样本中，鉴定出六种不同的 spp.（、、、 和 ）。我们的数据表明，PCR-RFLP检测是一种实用的 spp. 鉴定方法，能够与其他 （SIG） 物种以及犬类重要葡萄球菌病原体进行区分鉴定。耐药 spp. 分离株中，对四环素耐药的频率最高（21/34；61.76%；-值0.003），其次是庆大霉素（20/34；58.82%）和卡那霉素（20/34；58.82%）。
这些结果对于指导兽医学中抗生素的合理使用至关重要。它们还将有助于设计有效的控制策略并评估其有效性。
