Zang Yu, Chen Jun, Li Ruoxi, Shang Shuai, Tang Xuexi
College of Marine Life Sciences, Ocean University of China, Qingdao, China.
Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
PeerJ. 2020 May 5;8:e9063. doi: 10.7717/peerj.9063. eCollection 2020.
Superoxide dismutases (SODs) serve as the first line of defense in the plant antioxidant enzyme system, and play a primary role in the removal of reactive oxygen species (ROS). However, our understanding of the functions of the SOD family in is limited. In this study, a systematic analysis was conducted on the characteristics of the SOD genes in at the whole-genome level. Five SOD genes were identified, consisting of two , two , and one . Phylogenetic analysis showed that proteins could be divided into two major categories ( and ). Sequence motifs, gene structure, and the 3D-modeled protein structures further supported the phylogenetic analysis, with each subgroup having similar motifs, exon-intron structures, and protein structures. Additionally, several cis-elements were identified that may respond to biotic and abiotic stresses. Transcriptome analysis revealed expression diversity of in various tissues. Moreover, qRT-PCR analysis showed that the expression level of most genes trended to decreased expression with the increase of temperature, indicating that heat stress inhibits expression of and may result in reduced ability of to scavenge ROS. Our results provide a basis for further functional research on the SOD gene family in , which will help to determine the molecular mechanism of genes in response to environmental stress.
超氧化物歧化酶（SODs）是植物抗氧化酶系统中的第一道防线，在清除活性氧（ROS）方面发挥着主要作用。然而，我们对[具体植物名称]中SOD家族功能的了解有限。在本研究中，对[具体植物名称]的SOD基因特征进行了全基因组水平的系统分析。共鉴定出5个SOD基因，包括2个[具体类型1]、2个[具体类型2]和1个[具体类型3]。系统发育分析表明，[具体植物名称]的SOD蛋白可分为两大类（[具体类别1]和[具体类别2]）。序列基序、基因结构和三维建模的蛋白质结构进一步支持了系统发育分析，每个亚组具有相似的基序、外显子-内含子结构和蛋白质结构。此外，还鉴定出了几个可能响应生物和非生物胁迫的顺式元件。转录组分析揭示了[具体植物名称]中SOD基因在不同组织中的表达多样性。此外，qRT-PCR分析表明，大多数SOD基因的表达水平随着温度的升高而呈下降趋势，这表明热胁迫抑制了[具体植物名称]中SOD基因的表达，并可能导致其清除ROS的能力降低。我们的研究结果为进一步研究[具体植物名称]中SOD基因家族的功能提供了基础，这将有助于确定[具体植物名称]中SOD基因响应环境胁迫的分子机制。
