Żabicka Justyna, Kirschey Tom, Migdałek Grzegorz, Słomka Aneta, Kuta Elżbieta
Department of Plant Cytology and Embryology, Institute of Botany, Faculty of Biology, Jagiellonian University in Kraków, 9 Gronostajowa St., 30-387 Cracow, Poland.
International Peatland and Southeast Asia Programme, International Department, The Nature and Biodiversity Conservation Union (NABU), 3 Charitéstrasse, 10117 Berlin, Germany.
Biology (Basel). 2023 Feb 24;12(3):362. doi: 10.3390/biology12030362.
In Europe, the - group comprises Ledeb., L., (Kuta) G. H. Loos (= subsp. Kuta), interspecific hybrids, and putative introgressants. The genetic affinity of to , and their shared origin via hybridization followed by polyploidization, were confirmed using inter simple sequence repeat (ISSR) markers, restriction site-associated DNA sequencing (RAD-Seq), and a low-copy nuclear gene, , which encodes glucose-6-phosphate isomerase. The other taxa of subsect. were not identified as putative parents of by . Our analyses indicated that can be included in the morphological and genetic variation of . The ISSR, RAD-Seq, and genome size value separated well from and hybrids. The results also reopen the discussion on intraspecific variation in the context of taxa ranks and species concepts. The reduced tolerance of in Europe to changing environmental conditions might result from low genetic differentiation and heterozygosity, as well as the increased number of interspecific hybrids ( × ), and eventually can possibly lead to its extinction. The disappearance of populations/individuals of this species may indicate anthropogenic changes occurring in peatlands.
在欧洲，该类群包括 Ledeb.、L.、(Kuta) G. H. Loos（= Kuta 亚种）、种间杂种以及推定的渗入种。通过简单序列重复区间（ISSR）标记、限制性位点关联 DNA 测序（RAD-Seq）以及一个编码葡萄糖-6-磷酸异构酶的低拷贝核基因，证实了该类群与其他类群的遗传亲缘关系，以及它们通过杂交后多倍体化的共同起源。该亚组的其他分类群未被确定为该类群的推定亲本。我们的分析表明，该类群可包含在其他类群的形态和遗传变异中。ISSR、RAD-Seq 和基因组大小值很好地将该类群与其他类群及杂种区分开来。研究结果还重新开启了关于分类等级和物种概念背景下种内变异的讨论。欧洲该类群对不断变化的环境条件耐受性降低，可能是由于遗传分化和杂合性较低，以及种间杂种（× ）数量增加，最终可能导致其灭绝。该物种种群/个体的消失可能表明泥炭地正在发生人为变化。
