Department of Chemical and Biological Engineering, Gachon University, 1342 Seongnamdaero, Seongnam-si 13120, Republic of Korea.
Biosensors (Basel). 2023 Feb 22;13(3):308. doi: 10.3390/bios13030308.
Dopamine (DA), an organic biomolecule that acts as both a hormone and a neurotransmitter, is essential in regulating emotions and metabolism in living organisms. The accurate determination of DA is important because it indicates early signs of serious neurological disorders. Covalent organic frameworks (COFs) and metal-organic frameworks (MOFs) have received considerable attention in recent years as promising porous materials with an unrivaled degree of tunability for electrochemical biosensing applications. This study adopted a solvothermal strategy for the synthesis of a conjugated microporous poly(arylene ethynylene)-4 (CMP-4) network using the Sonagashira-Hagihara cross-coupling reaction. To increase the crystallinity and electrical conductivity of the material, CMP-4 was enveloped around carbon nanotubes (CNTs), followed by iodine doping. When used as an electrochemical probe, the as-synthesized material (I-CMP-CNT-4) exhibited excellent selectivity and sensitivity to dopamine in the phosphate-buffered solution. The detection limits of the electrochemical sensor were 1 and 1.7 μM based on cyclic voltammetry (CV) and differential pulse voltammetry (DPV).
多巴胺（DA）是一种有机生物分子，既是激素又是神经递质，对调节生物体内的情绪和新陈代谢至关重要。准确测定多巴胺很重要，因为它表明了严重神经紊乱的早期迹象。共价有机框架（COFs）和金属有机框架（MOFs）作为具有无与伦比的电化学生物传感应用可调性的有前途的多孔材料，近年来受到了极大的关注。本研究采用溶剂热策略，通过 Sonagashira-Hagihara 交叉偶联反应合成了一种共轭微孔聚（亚芳基乙炔）-4（CMP-4）网络。为了提高材料的结晶度和电导率，CMP-4 被包裹在碳纳米管（CNTs）周围，然后进行碘掺杂。作为电化学探针使用时，合成的材料（I-CMP-CNT-4）在磷酸盐缓冲溶液中对多巴胺表现出优异的选择性和灵敏度。电化学传感器的检测限基于循环伏安法（CV）和差分脉冲伏安法（DPV）分别为 1 和 1.7 μM。
