• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

从布加勒斯特社区获得性感染中分离出的 spp. 耐药性和毒力标记物的表型和基因型特征，以及银纳米颗粒对高耐药菌株的影响。

Phenotypic and Genotypic Characterization of Resistance and Virulence Markers in spp. Isolated from Community-Acquired Infections in Bucharest, and the Impact of AgNPs on the Highly Resistant Isolates.

作者信息

Corbu Viorica Maria, Georgescu Ana-Maria, Marinas Ioana Cristina, Pericleanu Radu, Mogos Denisa Vasilica, Dumbravă Andreea Ștefania, Marinescu Liliana, Pecete Ionut, Vassu-Dimov Tatiana, Czobor Barbu Ilda, Csutak Ortansa, Ficai Denisa, Gheorghe-Barbu Irina

机构信息

Faculty of Biology, University of Bucharest, Intrarea Portocalelor No. 1-3, 060101 Bucharest, Romania.

The Research Institute of the University of Bucharest (ICUB), 050095 Bucharest, Romania.

出版信息

J Fungi (Basel). 2024 Aug 9;10(8):563. doi: 10.3390/jof10080563.

DOI:10.3390/jof10080563
PMID:39194889
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11355189/
Abstract

BACKGROUND

This study aimed to determine, at the phenotypic and molecular levels, resistance and virulence markers in spp. isolated from community-acquired infections in Bucharest outpatients during 2021, and to demonstrate the efficiency of alternative solutions against them based on silver nanoparticles (AgNPs).

METHODS

A total of 62 spp. strains were isolated from dermatomycoses and identified using chromogenic culture media and MALDI-TOF MS, and then investigated for their antimicrobial resistance and virulence markers (VMs), as well as for metabolic enzymes using enzymatic tests for the expression of soluble virulence factors, their biofilm formation and adherence capacity on HeLa cells, and PCR assays for the detection of virulence markers and the antimicrobial activity of alternative solutions based on AgNPs.

RESULTS

Of the total of 62 strains, 45.16% were ; 29.03% ; 9.67% ; 3.22% , and ; and 1.66% , , , and . Aesculin hydrolysis, caseinase, and amylase production were detected in the analyzed strains. The strains exhibited different indices of adherence to HeLa cells and were positive in decreasing frequency order for the , , and , genes (/). An inhibitory effect on microbial growth, adherence capacity, and on the production of virulence factors was obtained using AgNPs.

CONCLUSIONS

The obtained results in and non- circulating in Bucharest outpatients were characterized by moderate-to-high potential to produce VMs, necessitating epidemiological surveillance measures to minimize the chances of severe invasive infections.

摘要

背景

本研究旨在从表型和分子水平确定2021年布加勒斯特门诊患者社区获得性感染分离出的[具体菌种]中的耐药性和毒力标志物，并证明基于银纳米颗粒（AgNPs）的替代解决方案对它们的有效性。

方法

从皮肤真菌病中分离出总共62株[具体菌种]菌株，使用显色培养基和基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）进行鉴定，然后研究它们的抗菌耐药性和毒力标志物（VMs），以及使用酶促试验检测可溶性毒力因子表达的代谢酶、它们在HeLa细胞上的生物膜形成和黏附能力，以及用于检测毒力标志物和基于AgNPs的替代解决方案抗菌活性的PCR分析。

结果

在总共62株菌株中，45.16%为[具体菌种1]；29.03%为[具体菌种2]；9.67%为[具体菌种3]；3.22%为[具体菌种4]、[具体菌种5]和[具体菌种6]；1.66%为[具体菌种7]、[具体菌种8]、[具体菌种9]和[具体菌种10]。在所分析的菌株中检测到七叶苷水解、酪蛋白酶和淀粉酶产生。这些菌株对HeLa细胞表现出不同的黏附指数，并且[具体基因1]、[具体基因2]和[具体基因3]基因（/）呈阳性，频率递减。使用AgNPs对微生物生长、黏附能力和毒力因子产生具有抑制作用。

结论

在布加勒斯特门诊患者中未传播的[具体菌种1]和[具体菌种2]中获得的结果具有产生VMs的中到高潜力，需要采取流行病学监测措施以尽量减少严重侵袭性感染的机会。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/130a/11355189/06f097dda360/jof-10-00563-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/130a/11355189/e1c8412ff9b4/jof-10-00563-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/130a/11355189/5a1e7d0c72af/jof-10-00563-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/130a/11355189/356c3b02d1e0/jof-10-00563-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/130a/11355189/59534cf6583c/jof-10-00563-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/130a/11355189/69438abcd43b/jof-10-00563-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/130a/11355189/a19734467314/jof-10-00563-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/130a/11355189/06f097dda360/jof-10-00563-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/130a/11355189/e1c8412ff9b4/jof-10-00563-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/130a/11355189/5a1e7d0c72af/jof-10-00563-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/130a/11355189/356c3b02d1e0/jof-10-00563-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/130a/11355189/59534cf6583c/jof-10-00563-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/130a/11355189/69438abcd43b/jof-10-00563-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/130a/11355189/a19734467314/jof-10-00563-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/130a/11355189/06f097dda360/jof-10-00563-g007.jpg

相似文献

1
Phenotypic and Genotypic Characterization of Resistance and Virulence Markers in spp. Isolated from Community-Acquired Infections in Bucharest, and the Impact of AgNPs on the Highly Resistant Isolates.从布加勒斯特社区获得性感染中分离出的 spp. 耐药性和毒力标记物的表型和基因型特征，以及银纳米颗粒对高耐药菌株的影响。
J Fungi (Basel). 2024 Aug 9;10(8):563. doi: 10.3390/jof10080563.
2
Molecular Epidemiology, Antifungal Susceptibility, and Virulence Evaluation of Isolates Causing Invasive Infection in a Tertiary Care Teaching Hospital.分子流行病学、抗真菌药敏试验和毒力评估在一所三级教学医院引起侵袭性感染的分离株。
Front Cell Infect Microbiol. 2021 Sep 15;11:721439. doi: 10.3389/fcimb.2021.721439. eCollection 2021.
3
Phenotypic and genotypic characterization of virulence markers and antifungal susceptibility of oral Candida species from diabetic and non-diabetic hemodialysis patients.从糖尿病和非糖尿病血液透析患者的口腔念珠菌中进行表型和基因型毒力标志物特征分析及抗真菌药敏试验。
BMC Oral Health. 2023 May 4;23(1):261. doi: 10.1186/s12903-023-02970-8.
4
Candida and candidaemia. Susceptibility and epidemiology.念珠菌与念珠菌血症。药敏性与流行病学。
Dan Med J. 2013 Nov;60(11):B4698.
5
[Antifungal susceptibility profiles of Candida species to triazole: application of new CLSI species-specific clinical breakpoints and epidemiological cutoff values for characterization of antifungal resistance].念珠菌属对三唑类药物的抗真菌药敏谱：应用美国临床和实验室标准协会（CLSI）新的种特异性临床折点和流行病学截断值来表征抗真菌药物耐药性
Mikrobiyol Bul. 2016 Jan;50(1):122-32. doi: 10.5578/mb.10682.
6
Non-albicans Candida spp. causing fungaemia: pathogenicity and antifungal resistance.引起真菌血症的非白色念珠菌属：致病性与抗真菌耐药性
J Hosp Infect. 2002 Apr;50(4):243-60. doi: 10.1053/jhin.2001.1151.
7
[Investigation of antifungal susceptibilities and some virulence factors of Candida strains isolated from blood cultures and genotyping by RAPD-PCR].[从血培养中分离的念珠菌菌株的抗真菌药敏性及一些毒力因子研究与随机扩增多态性DNA聚合酶链反应基因分型]
Mikrobiyol Bul. 2011 Apr;45(2):306-17.
8
Characterization of Candida species isolated from the hands of the healthcare workers in the neonatal intensive care unit.从新生儿重症监护病房医护人员手部分离出的念珠菌属的鉴定
Med Mycol. 2019 Jul 1;57(5):588-594. doi: 10.1093/mmy/myy101.
9
Oral Candida albicans isolates from HIV-positive individuals have similar in vitro biofilm-forming ability and pathogenicity as invasive Candida isolates.从 HIV 阳性个体中分离出的口腔白色念珠菌与侵袭性念珠菌分离株具有相似的体外生物膜形成能力和致病性。
BMC Microbiol. 2011 Nov 4;11:247. doi: 10.1186/1471-2180-11-247.
10
Anticandidal activity of biosynthesized silver nanoparticles: effect on growth, cell morphology, and key virulence attributes of Candida species.生物合成银纳米粒子的抗真菌活性：对念珠菌属生长、细胞形态和关键毒力特性的影响。
Int J Nanomedicine. 2019 Jun 28;14:4667-4679. doi: 10.2147/IJN.S210449. eCollection 2019.

引用本文的文献

1
Multifunctional protective strategies for wooden cultural heritage: antimicrobial efficacy of polyacrylic resins, siloxane coupling agents, and silver nanoparticles.木质文化遗产的多功能保护策略：聚丙烯酸树脂、硅氧烷偶联剂和银纳米颗粒的抗菌效果
Front Microbiol. 2025 Aug 22;16:1642335. doi: 10.3389/fmicb.2025.1642335. eCollection 2025.
2
Antifungal and Antioxidant Activity of Thiourea Derivatives Against Nosocomial Strains Isolated in Romania.硫脲衍生物对罗马尼亚分离出的医院菌株的抗真菌和抗氧化活性
Molecules. 2025 Apr 9;30(8):1675. doi: 10.3390/molecules30081675.
3
Emerging Resistance and Virulence Patterns in : Insights into Silver Nanoparticles as an Antimicrobial Strategy.

本文引用的文献

1
Molecular Diagnostics for Invasive Fungal Diseases: Current and Future Approaches.侵袭性真菌病的分子诊断：现状与未来方法
J Fungi (Basel). 2024 Jun 26;10(7):447. doi: 10.3390/jof10070447.
2
Epidemiological patterns of candidaemia: A comprehensive analysis over a decade.念珠菌血症的流行病学模式：十年综合分析。
Mycoses. 2024 May;67(5):e13729. doi: 10.1111/myc.13729.
3
Evaluation of the Vitek 2 system for antifungal susceptibility testing of using a representative international panel of clinical isolates: overestimation of amphotericin B resistance and underestimation of fluconazole resistance.
新兴的耐药性和毒力模式：对银纳米颗粒作为抗菌策略的见解
Antibiotics (Basel). 2025 Jan 7;14(1):46. doi: 10.3390/antibiotics14010046.
采用代表性的国际临床分离株进行抗真菌药敏试验的 Vitek 2 系统评估：两性霉素 B 耐药性高估和氟康唑耐药性低估。
J Clin Microbiol. 2024 Apr 10;62(4):e0152823. doi: 10.1128/jcm.01528-23. Epub 2024 Mar 19.
4
The Effectiveness of Silver Nanoparticles Mixed with Calcium Hydroxide against : An Ex Vivo Analysis.混合氢氧化钙的银纳米颗粒的有效性：一项体外分析。
Microorganisms. 2024 Jan 29;12(2):289. doi: 10.3390/microorganisms12020289.
5
Molecular Mechanisms Associated with Antifungal Resistance in Pathogenic Species.与致病物种中抗真菌耐药性相关的分子机制。
Cells. 2023 Nov 19;12(22):2655. doi: 10.3390/cells12222655.
6
Candida albicans and Antifungal Peptides.白色念珠菌与抗真菌肽
Infect Dis Ther. 2023 Dec;12(12):2631-2648. doi: 10.1007/s40121-023-00889-9. Epub 2023 Nov 8.
7
Phenotypic and Genotypic Characterization of Recently Isolated Multidrug-Resistant Clinical and Aquatic Strains and Demonstration of Silver Nanoparticle Potency.近期分离的多药耐药临床菌株和水生菌株的表型与基因型特征以及银纳米颗粒效能的证明
Microorganisms. 2023 Sep 28;11(10):2439. doi: 10.3390/microorganisms11102439.
8
Snapshot of Phenotypic and Molecular Virulence and Resistance Profiles in Multidrug-Resistant Strains Isolated in a Tertiary Hospital in Romania.罗马尼亚一家三级医院分离出的多重耐药菌株的表型、分子毒力和耐药谱快照
Pathogens. 2023 Apr 17;12(4):609. doi: 10.3390/pathogens12040609.
9
Characterization of Virulence Factors in Species Causing Candidemia in a Tertiary Care Hospital in Bangkok, Thailand.泰国曼谷一家三级护理医院中引起念珠菌血症的物种的毒力因子特征分析。
J Fungi (Basel). 2023 Mar 14;9(3):353. doi: 10.3390/jof9030353.
10
Mechanisms of Antifungal Properties of Metal Nanoparticles.金属纳米颗粒的抗真菌特性机制
Nanomaterials (Basel). 2022 Dec 16;12(24):4470. doi: 10.3390/nano12244470.