Podunavac Ivana, Djocos Miroslav, Vejin Marija, Birgermajer Slobodan, Pavlovic Zoran, Kojic Sanja, Petrovic Bojan, Radonic Vasa
University of Novi Sad, BioSense Institute, Dr Zorana Đinđića 1, 21000 Novi Sad, Serbia.
University of Novi Sad, Faculty of Technical Sciences, Trg Dositeja Obradovića 6, 21000 Novi Sad, Serbia.
Micromachines (Basel). 2023 Feb 21;14(3):503. doi: 10.3390/mi14030503.
The connection of macrosystems with microsystems for in-line measurements is important in different biotechnological processes as it enables precise and accurate monitoring of process parameters at a small scale, which can provide valuable insights into the process, and ultimately lead to improved process control and optimization. Additionally, it allows continuous monitoring without the need for manual sampling and analysis, leading to more efficient and cost-effective production. In this paper, a 3D printed microfluidic (MF) chip for glucose (Glc) sensing in a liquid analyte is proposed. The chip made in Poly(methyl methacrylate) (PMMA) contains integrated serpentine-based micromixers realized via stereolithography with a slot for USB-like integration of commercial DropSens electrodes. After adjusting the sample's pH in the first micromixer, small volumes of the sample and enzyme are mixed in the second micromixer and lead to a sensing chamber where the Glc concentration is measured via chronoamperometry. The sensing potential was examined for Glc concentrations in acetate buffer in the range of 0.1-100 mg/mL and afterward tested for Glc sensing in a cell culturing medium. The proposed chip showed great potential for connection with macrosystems, such as bioreactors, for direct in-line monitoring of a quality parameter in a liquid sample.
宏系统与微系统的连接用于在线测量，在不同的生物技术过程中至关重要，因为它能够在小尺度上精确、准确地监测过程参数，这可为过程提供有价值的见解，并最终实现更好的过程控制和优化。此外，它无需人工采样和分析即可进行连续监测，从而实现更高效、更具成本效益的生产。本文提出了一种用于液体分析物中葡萄糖（Glc）传感的3D打印微流控（MF）芯片。该芯片由聚甲基丙烯酸甲酯（PMMA）制成，包含通过立体光刻实现的集成蛇形微混合器，并带有一个用于类似USB集成商用DropSens电极的插槽。在第一个微混合器中调节样品的pH值后，小体积的样品和酶在第二个微混合器中混合，然后进入传感腔室，通过计时电流法测量Glc浓度。对醋酸盐缓冲液中0.1 - 100 mg/mL范围内的Glc浓度检测了传感电位，随后在细胞培养基中测试了Glc传感。所提出的芯片显示出与生物反应器等宏系统连接的巨大潜力，可用于直接在线监测液体样品中的质量参数。
