Faye Lindiwe M, Hosu Mojisola C, Vasaikar Sandeep, Dippenaar Anzaan, Oostvogels Selien, Warren Rob M, Apalata Teke
Department of Laboratory Medicine and Pathology, Walter Sisulu University and National Health Laboratory Services (NHLS), Private Bag X5117, Mthatha 5099, South Africa.
Family Medicine and Population Health (FAMPOP), Faculty of Medicine and Health Sciences, University of Antwerp, BE-2000 Antwerp, Belgium.
Pathogens. 2023 Mar 17;12(3):475. doi: 10.3390/pathogens12030475.
Tuberculosis (TB), an infectious airborne disease caused by (Mtb), is a serious public health threat reported as the leading cause of morbidity and mortality worldwide. South Africa is a high-TB-burden country with TB being the highest infectious disease killer. This study investigated the distribution of Mtb mutations and spoligotypes in rural Eastern Cape Province. The Mtb isolates included were 1157 from DR-TB patients and analysed by LPA followed by spoligotyping of 441 isolates. The distribution of mutations and spoligotypes was done by spatial analysis. The gene had the highest number of mutations. The distribution of and mutations was more prevalent in four healthcare facilities, mutations were more prevalent in three healthcare facilities, and heteroresistant isolates were more prevalent in five healthcare facilities. The Mtb was genetically diverse with Beijing more prevalent and largely distributed. Spatial analysis and mapping of gene mutations and spoligotypes revealed a better picture of distribution.
结核病（TB）是由结核分枝杆菌（Mtb）引起的一种空气传播的传染病，是一种严重的公共卫生威胁，据报告是全球发病和死亡的主要原因。南非是一个结核病负担沉重的国家，结核病是头号传染病杀手。本研究调查了东开普省农村地区Mtb突变和 spoligotypes 的分布情况。纳入的Mtb分离株来自1157例耐多药结核病患者，并通过线性探针分析（LPA）进行分析，随后对441株分离株进行spoligotyping分析。通过空间分析确定突变和spoligotypes的分布情况。该基因的突变数量最多。katG和rpoB突变在四个医疗机构中更为普遍，pncA突变在三个医疗机构中更为普遍，异耐药分离株在五个医疗机构中更为普遍。Mtb具有遗传多样性，北京基因型更为普遍且分布广泛。基因突变和spoligotypes的空间分析和绘图揭示了更好的分布情况。
