• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

含乳酸杆菌菌株的羟乙基纤维素基凝胶的研制与表征：体外和离体模型中的抗菌效果评估

Development and Characterization of Hydroxyethyl Cellulose-Based Gels Containing Lactobacilli Strains: Evaluation of Antimicrobial Effects in In Vitro and Ex Vivo Models.

作者信息

Sousa Marcela Almeida Dos Santos de, Ferreira Alexia Figueiredo, da Silva Camila Caetano, Silva Marcos Andrade, Bazan Tamyris Alicely Xavier Nogueira, Monteiro Cristina de Andrade, Monteiro Andrea de Souza, Sousa Joicy Cortez de Sá, da Silva Luís Cláudio Nascimento, Zagmignan Adrielle

机构信息

Laboratory of Microbial Pathogenesis Patogenicidade Microbiana, CEUMA University, São Luís 65075-120, Brazil.

Laboratory of Odontology, CEUMA University, São Luís 65075-120, Brazil.

出版信息

Pharmaceuticals (Basel). 2023 Mar 22;16(3):468. doi: 10.3390/ph16030468.

DOI:10.3390/ph16030468
PMID:36986568
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10058878/
Abstract

This study aimed to develop a hydroxyethyl cellulose-based topical formulation containing probiotics and to evaluate its antimicrobial action using in vivo and ex vivo models. Initially, the antagonistic effects of ATCC 10863, ATCC 23271, ATCC 8014 and LP-G18-A11 were analyzed against ATCC 29212, ATCC 700603, ATCC 27853 and ATCC 2785. The best action was seen for LP-G18-A11, which presented high inhibition against and . Then, lactobacilli strains were incorporated into hydroxyethyl cellulose-based gels (natrosol); however, only the LP-G18-A11-incorporated gels (5% and 3%) showed antimicrobial effects. The LP-G18-A11 gel (5%) maintained its antimicrobial effects and viability up to 14 and 90 days at 25 °C and 4 °C, respectively. In the ex vivo assay using porcine skin, the LP-G18-A11 gel (5%) significantly reduced the skin loads of and after 24 h, while only was reduced after 72 h. Moreover, the LP-G18-A11 gel (5%) showed stability in the preliminary and accelerated assays. Taken together, the results show the antimicrobial potential of LP-G18-A11, which may be applied in the development of new dressings for the treatment of infected wounds.

摘要

本研究旨在开发一种含有益生菌的羟乙基纤维素基局部用制剂，并使用体内和体外模型评估其抗菌作用。最初，分析了ATCC 10863、ATCC 23271、ATCC 8014和LP-G18-A11对ATCC 29212、ATCC 700603、ATCC 27853和ATCC 2785的拮抗作用。LP-G18-A11表现出最佳作用，对[具体菌株1]和[具体菌株2]呈现出高抑制作用。然后，将乳酸杆菌菌株掺入羟乙基纤维素基凝胶（羟丙基甲基纤维素）中；然而，只有掺入LP-G18-A11的凝胶（5%和3%）显示出抗菌效果。LP-G18-A11凝胶（5%）在25℃和4℃下分别在14天和90天内保持其抗菌效果和活力。在使用猪皮的体外试验中，LP-G18-A11凝胶（5%）在24小时后显著降低了[具体菌株3]和[具体菌株4]的皮肤载量，而在72小时后仅[具体菌株4]的载量降低。此外，LP-G18-A11凝胶（5%）在初步试验和加速试验中显示出稳定性。综上所述，结果表明LP-G18-A11具有抗菌潜力，可应用于开发治疗感染伤口的新型敷料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a80c/10058878/dfdb4f24eaa7/pharmaceuticals-16-00468-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a80c/10058878/a93556e115fc/pharmaceuticals-16-00468-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a80c/10058878/029b742423fb/pharmaceuticals-16-00468-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a80c/10058878/dfdb4f24eaa7/pharmaceuticals-16-00468-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a80c/10058878/a93556e115fc/pharmaceuticals-16-00468-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a80c/10058878/029b742423fb/pharmaceuticals-16-00468-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a80c/10058878/dfdb4f24eaa7/pharmaceuticals-16-00468-g003.jpg

相似文献

1
Development and Characterization of Hydroxyethyl Cellulose-Based Gels Containing Lactobacilli Strains: Evaluation of Antimicrobial Effects in In Vitro and Ex Vivo Models.含乳酸杆菌菌株的羟乙基纤维素基凝胶的研制与表征：体外和离体模型中的抗菌效果评估
Pharmaceuticals (Basel). 2023 Mar 22;16(3):468. doi: 10.3390/ph16030468.
2
The Potential of ATCC 14917 in the Development of Alginate-Based Gel Formulations with Anti- Properties.ATCC 14917在开发具有抗菌特性的藻酸盐基凝胶制剂中的潜力。
Pharmaceuticals (Basel). 2023 Aug 7;16(8):1112. doi: 10.3390/ph16081112.
3
Selective utilization of gluco-oligosaccharides by lactobacilli: A mechanism study revealing the impact of glycosidic linkages and degree of polymerization on their utilization.乳酸菌对低聚糖的选择性利用：揭示糖苷键和聚合度对其利用影响的机制研究。
J Food Sci. 2024 Jan;89(1):523-539. doi: 10.1111/1750-3841.16851. Epub 2023 Nov 27.
4
Topical Administration of Accelerates the Healing of Chronic Diabetic Foot Ulcers through Modifications of Infection, Angiogenesis, Macrophage Phenotype and Neutrophil Response.局部给药通过改变感染、血管生成、巨噬细胞表型和中性粒细胞反应加速慢性糖尿病足溃疡的愈合。
Microorganisms. 2022 Mar 17;10(3):634. doi: 10.3390/microorganisms10030634.
5
Effectiveness of the two microorganisms Lactobacillus fermentum LF15 and Lactobacillus plantarum LP01, formulated in slow-release vaginal tablets, in women affected by bacterial vaginosis: a pilot study.两种微生物发酵乳杆菌LF15和植物乳杆菌LP01制成缓释阴道片，对患有细菌性阴道病的女性的有效性：一项试点研究。
J Clin Gastroenterol. 2014 Nov-Dec;48 Suppl 1:S106-12. doi: 10.1097/MCG.0000000000000226.
6
Antimicrobial potential for the combination of bovine lactoferrin or its hydrolysate with lactoferrin-resistant probiotics against foodborne pathogens.牛乳铁蛋白或其水解物与乳铁蛋白抗性益生菌组合对食源性病原体的抗菌潜力。
J Dairy Sci. 2013 Mar;96(3):1438-46. doi: 10.3168/jds.2012-6112. Epub 2013 Jan 16.
7
Good Bugs vs Bad Bugs: Evaluation of Inhibitory Effect of Selected Probiotics against Enterococcus faecalis.有益菌与有害菌：所选益生菌对粪肠球菌抑制作用的评估
J Contemp Dent Pract. 2017 Apr 1;18(4):312-316. doi: 10.5005/jp-journals-10024-2037.
8
ATCC 23271 Displays Inhibitory Activities against spp.美国典型培养物保藏中心23271对……显示出抑制活性
Front Microbiol. 2016 Oct 27;7:1722. doi: 10.3389/fmicb.2016.01722. eCollection 2016.
9
Repression of and by Strain AG10 in In Vivo Model.体内模型中AG10菌株对……的抑制作用 （原文中“Repression of and by ”部分内容缺失，无法完整准确翻译）
Microorganisms. 2023 May 16;11(5):1297. doi: 10.3390/microorganisms11051297.
10
Probiotic Potentiality from Versatile Strains as Resource to Enhance Freshwater Fish Health.多功能菌株作为增强淡水鱼健康资源的益生菌潜力
Microorganisms. 2022 Feb 17;10(2):463. doi: 10.3390/microorganisms10020463.

引用本文的文献

1
Next-Generation Wound Care: A Scoping Review on Probiotic, Prebiotic, Synbiotic, and Postbiotic Cutaneous Formulations.下一代伤口护理：关于益生菌、益生元、合生元和后生元皮肤制剂的范围综述。
Pharmaceuticals (Basel). 2025 May 9;18(5):704. doi: 10.3390/ph18050704.
2
Hydrogel Containing Biogenic Silver Nanoparticles and Essential Oil for Burn Wounds: Antimicrobial Efficacy Using Ex Vivo and In Vivo Methods Against Multidrug-Resistant Microorganisms.含生物源银纳米颗粒和精油的水凝胶用于烧伤创面：采用体外和体内方法对多重耐药微生物的抗菌效果
Pharmaceutics. 2025 Apr 10;17(4):503. doi: 10.3390/pharmaceutics17040503.
3
Global Trends and Scientific Impact of Topical Probiotics in Dermatological Treatment and Skincare.

本文引用的文献

1
Effect of bleaching gels with different thickeners under normal and hyposalivation conditions: in situ study.不同稠度剂的漂白凝胶在正常和低唾液分泌条件下的效果：原位研究。
J Appl Oral Sci. 2022 Dec 2;30:e20220285. doi: 10.1590/1678-7757-2022-0285. eCollection 2022.
2
Hydrogel and Effects of Crosslinking Agent on Cellulose-Based Hydrogels: A Review.水凝胶及交联剂对纤维素基水凝胶的影响：综述
Gels. 2022 Sep 7;8(9):568. doi: 10.3390/gels8090568.
3
Progress in Hydrogels for Skin Wound Repair.水凝胶在皮肤创伤修复中的研究进展。
局部用益生菌在皮肤病治疗和皮肤护理中的全球趋势及科学影响
Microorganisms. 2024 Oct 3;12(10):2010. doi: 10.3390/microorganisms12102010.
4
The Potential of ATCC 14917 in the Development of Alginate-Based Gel Formulations with Anti- Properties.ATCC 14917在开发具有抗菌特性的藻酸盐基凝胶制剂中的潜力。
Pharmaceuticals (Basel). 2023 Aug 7;16(8):1112. doi: 10.3390/ph16081112.
Macromol Biosci. 2022 Jul;22(7):e2100475. doi: 10.1002/mabi.202100475. Epub 2022 Apr 14.
4
Topical Probiotics: More Than a Skin Deep.局部用益生菌：不止于表面
Pharmaceutics. 2022 Mar 3;14(3):557. doi: 10.3390/pharmaceutics14030557.
5
Spray dried lactobacilli maintain viability and feruloyl esterase activity during prolonged storage and under gastrointestinal tract conditions.喷雾干燥的乳酸菌在长期储存和胃肠道条件下保持活力和阿魏酸酯酶活性。
J Food Sci Technol. 2022 Mar;59(3):1202-1210. doi: 10.1007/s13197-021-05125-1. Epub 2021 May 6.
6
Living Bacterial Hydrogels for Accelerated Infected Wound Healing.用于加速感染性伤口愈合的活体细菌水凝胶。
Adv Sci (Weinh). 2021 Dec;8(24):e2102545. doi: 10.1002/advs.202102545. Epub 2021 Oct 31.
7
An Insight into Biomolecules for the Treatment of Skin Infectious Diseases.用于治疗皮肤传染病的生物分子洞察
Pharmaceutics. 2021 Jul 2;13(7):1012. doi: 10.3390/pharmaceutics13071012.
8
Evaluation of Growth, Viability, Lactic Acid Production and Anti-Infective Effects of ATCC 9595 in Bacuri Juice ().ATCC 9595在巴西栗果汁中的生长、活力、乳酸产生及抗感染作用评估()。 （注：括号部分原文缺失具体内容）
Foods. 2021 Mar 12;10(3):603. doi: 10.3390/foods10030603.
9
Development and Characterization of Probiotic Lysate-Treated Chitosan Nanogel as a Novel Biocompatible Formulation for Wound Healing.益生菌酶解物处理壳聚糖纳米凝胶的研制及特性：一种新型的生物相容的伤口愈合制剂。
Biomed Res Int. 2020 Dec 28;2020:8868618. doi: 10.1155/2020/8868618. eCollection 2020.
10
Interplay between ESKAPE Pathogens and Immunity in Skin Infections: An Overview of the Major Determinants of Virulence and Antibiotic Resistance.皮肤感染中ESKAPE病原体与免疫的相互作用：毒力和抗生素耐药性主要决定因素概述
Pathogens. 2021 Feb 2;10(2):148. doi: 10.3390/pathogens10020148.