Sodré Monaliza Teresa Campos, Ferraz Fernanda Avelino, Alencar Amanda Karoline Vieira, Silva Karinny Farias, Silva Douglas Henrique Dos Santos, Silva Lucas Dos Santos, Araújo Carneiro Jéssica Silva Dos Santos, Monteiro Cristina Andrade, Silva Luis Cláudio Nascimento, Monteiro Andrea de Souza
Laboratory of Applied Microbiology, CEUMA University, São Luís 65075-120, MA, Brazil.
Laboratory of Microbial Pathogenesis, CEUMA University, São Luís 65075-120, MA, Brazil.
Pharmaceuticals (Basel). 2023 Aug 7;16(8):1112. doi: 10.3390/ph16081112.
This study aimed to evaluate the potential of lactic acid bacteria (LAB) in developing alginate-based gel formulations to inhibit . Initially, the antagonistic actions of three lactic acid bacteria (LAB) ( ATCC 10863, ATCC 14917, ATCC 23271) were evaluated against ATCC 25923. All tested LAB inhibited , but the highest activity was observed for ATCC 14917 ( < 0.05). The antimicrobial effects of ATCC 14917 cell suspensions, sonicate cells extract, and cell-free supernatants (pH 5 or 7) were analyzed using a broth-based assay. The cell suspensions inhibited at concentrations ≥ 10%, and these effects were confirmed by a time-kill assay. Alginate-based gels were formulated with cell suspensions, sonicate cells extract, and cell-free supernatant (pH 5). These formulations inhibited growth. Based on the results, the alginate gel with cell suspensions at 10% was selected for further characterization. ATCC 14917 survived in the alginate-based gel, especially when stored at 5 °C. At this temperature, the -containing alginate gel was stable, and it was in compliance with microbiological standards. These findings suggest it can be a promising agent for the topical treatment of infections induced by .
本研究旨在评估乳酸菌（LAB）在开发基于藻酸盐的凝胶制剂以抑制[此处原文有缺失]方面的潜力。最初，评估了三种乳酸菌（LAB）（ATCC 10863、ATCC 14917、ATCC 23271）对ATCC 25923的拮抗作用。所有测试的LAB均能抑制[此处原文有缺失]，但观察到ATCC 14917的活性最高（P<0.05）。使用基于肉汤的试验分析了ATCC 14917细胞悬液、超声破碎细胞提取物和无细胞上清液（pH 5或7）的抗菌效果。细胞悬液在浓度≥10%时能抑制[此处原文有缺失]，这些效果通过时间杀灭试验得到证实。用细胞悬液、超声破碎细胞提取物和无细胞上清液（pH 5）配制了基于藻酸盐的凝胶。这些制剂抑制了[此处原文有缺失]的生长。基于结果，选择了含10%细胞悬液的藻酸盐凝胶进行进一步表征。ATCC 14917在基于藻酸盐的凝胶中存活，尤其是在5℃储存时。在此温度下，含[此处原文有缺失]的藻酸盐凝胶稳定，且符合微生物标准。这些发现表明它可能是治疗由[此处原文有缺失]引起的感染的一种有前景的局部治疗剂。
