This study aimed to evaluate the potential of lactic acid bacteria (LAB) in developing alginate-based gel formulations to inhibit . Initially, the antagonistic actions of three lactic acid bacteria (LAB) ( ATCC 10863, ATCC 14917, ATCC 23271) were evaluated against ATCC 25923. All tested LAB inhibited , but the highest activity was observed for ATCC 14917 ( < 0.05). The antimicrobial effects of ATCC 14917 cell suspensions, sonicate cells extract, and cell-free supernatants (pH 5 or 7) were analyzed using a broth-based assay. The cell suspensions inhibited at concentrations ≥ 10%, and these effects were confirmed by a time-kill assay. Alginate-based gels were formulated with cell suspensions, sonicate cells extract, and cell-free supernatant (pH 5). These formulations inhibited growth. Based on the results, the alginate gel with cell suspensions at 10% was selected for further characterization. ATCC 14917 survived in the alginate-based gel, especially when stored at 5 °C. At this temperature, the -containing alginate gel was stable, and it was in compliance with microbiological standards. These findings suggest it can be a promising agent for the topical treatment of infections induced by .