Souiri Amal, Lemriss Sanaâ, El Maliki Bouchra, Falahi Hamadi, El Fahime Elmostafa, El Kabbaj Saâd
Laboratory of Research and Medical Analysis of Gendarmerie Royale, Department of Biosafety PCL3, Rabat 10100, Morocco.
Faculty of Medecine and Pharmacy, University Hassan II, Casablanca 20250, Morocco.
Vaccines (Basel). 2023 Mar 3;11(3):590. doi: 10.3390/vaccines11030590.
SARS-CoV-2 has caused a huge pandemic affecting millions of people and resulting innumerous deaths. A better understanding of the correlation between binding antibodies and neutralizing antibodies is necessary to address protective immunity post-infection or vaccination. Here, we investigate the humoral immune response and the seroprevalence of neutralizing antibodies following vaccination with adenovirus-based vector in 177 serum samples. A Microneutralization (MN) assay was used as a reference method to assess whether neutralizing antibody titers correlated with a positive signal in two commercially available serological tests:a rapid lateral flow immune-chromatographic assay (LFIA) and an enzyme-linked Fluorescence Assay (ELFA). Neutralizing antibodies were detected in most serum samples (84%). COVID-19 convalescent individuals showed high antibody titers and significant neutralizing activity. Spearman correlation coefficients between the serological and neutralization results ranged from 0.8 to 0.9, suggesting a moderate to strong correlation between commercial immunoassays test results (LFIA and ELFA) and virus neutralization.
严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引发了一场巨大的全球大流行，影响了数百万人并导致无数人死亡。为了解决感染后或接种疫苗后的保护性免疫问题，有必要更好地理解结合抗体与中和抗体之间的相关性。在此，我们对177份血清样本进行研究，调查基于腺病毒载体接种疫苗后的体液免疫反应和中和抗体的血清流行率。微量中和（MN）试验用作参考方法，以评估中和抗体滴度是否与两种市售血清学检测中的阳性信号相关：快速侧向流动免疫层析试验（LFIA）和酶联荧光试验（ELFA）。大多数血清样本（84%）中检测到了中和抗体。新冠康复者显示出高抗体滴度和显著的中和活性。血清学检测结果与中和结果之间的斯皮尔曼相关系数在0.8至0.9之间，表明市售免疫分析检测结果（LFIA和ELFA）与病毒中和之间存在中度至强相关性。
