Lee Eun Bee, Park Sooyeon, Kim Wonyong, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon, Republic of Korea.
Department of Microbiology, Chung-Ang University College of Medicine, 84 Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea.
Int J Syst Evol Microbiol. 2023 Mar;73(3). doi: 10.1099/ijsem.0.005794.
Two bacterial strains (designated as YSTF-M11 and TSTF-M6) were isolated from tidal flat sediments of the Yellow Sea, Republic of Korea, and taxonomically characterized. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain YSTF-M11 clusters with the type strains of species and strain TSTF-M6 clusters with the type strains of , and . Strains YSTF-M11 and TSTF-M6 exhibited 16S rRNA gene sequence similarity values of 97.5-98.9 % and 94.1-97.2 % to the type strains of four species and to the type strains of four species, respectively. An UBCG tree based on genomic sequences and a tree based on AAI showed that strains YSTF-M11 and TSTF-M6 form a cluster with the type strains of species and with the type strains of , and , respectively. The ANI and dDDH values between genomic sequences of strain YSTF-M11 and the type strains of four species and between those of strain TSTF-M6 and the type strains of the three species were in ranges of 74.0-75.9 and 18.2-19.7 % and 74.7-75.5 and 18.8-19.3 %, respectively. The DNA G+C contents of strains YSTF-M11 and TSTF-M6 were 60.3 and 61.9 % based on their genomic sequences. Both strains contained Q-10 as the predominant ubiquinone and C 7 as the major fatty acid. Strains YSTF-M11 and TSTF-M6 were separated from recognized species and , and , respectively, by their phenotypic properties together with the phylogenetic and genetic distinctiveness. Based on data presented in this study, strains YSTF-M11 (=KACC 21642 =NBRC 115155) and TSTF-M6 (=KACC 21643 =NBRC 115154) are considered to represent novel species of the genera and , respectively, for which the names sp. nov. and sp. nov. are proposed.
从大韩民国黄海潮滩沉积物中分离出两株细菌菌株（分别命名为YSTF-M11和TSTF-M6），并对其进行了分类学特征分析。基于16S rRNA基因序列构建的邻接法系统发育树显示，菌株YSTF-M11与 属物种的模式菌株聚类，菌株TSTF-M6与 属、 属和 属的模式菌株聚类。菌株YSTF-M11和TSTF-M6与4个 属物种的模式菌株以及与4个 属物种的模式菌株的16S rRNA基因序列相似性值分别为97.5 - 98.9%和94.1 - 97.2%。基于基因组序列构建的UBCG树和基于平均氨基酸一致性（AAI）构建的树表明菌株YSTF-M11和TSTF-M6分别与 属物种的模式菌株以及与 属、 属和 属的模式菌株形成一个聚类。菌株YSTF-M11的基因组序列与4个 属物种的模式菌株之间以及菌株TSTF-M6的基因组序列与3个 属物种的模式菌株之间的ANI（平均核苷酸一致性）和dDDH（数字化DNA-DNA杂交）值分别在74.0 - 75.9和18.2 - 19.7%以及74.7 - 75.5和18.8 - 用其基因组序列计算，菌株YSTF-M11和TSTF-M6的DNA G+C含量分别为60.3%和61.9%。两株菌均以Q-10作为主要泛醌，以C 7作为主要脂肪酸。菌株YSTF-M11和TSTF-M6通过其表型特征以及系统发育和遗传独特性分别与已确认的 属物种以及 属、 属和 属物种区分开来。基于本研究提供的数据，菌株YSTF-M11（=KACC 21642 =NBRC 115155）和TSTF-M6（=KACC 21643 =NBRC 115154）分别被认为代表 属和 属的新物种，为此提出新物种名称 属新种和 属新种。 （注：原文中部分属名未明确给出，用 代替）
