Fan Yanxiao, Gu Ronghui, Zhang Ruifei, Wang Miaomiao, Xu Heran, Wang Min, Long Chunlin
Key Laboratory of Ecology and Environment in Minority Areas (Minzu University of China), National Ethnic Affairs Commission, Beijing, China.
College of Life and Environmental Sciences, Minzu University of China, Beijing, China.
Front Pharmacol. 2023 Mar 15;14:1068849. doi: 10.3389/fphar.2023.1068849. eCollection 2023.
Bunge (Sapindaceae or formerly Aceraceae) is a tall deciduous tree native to China. Traditionally, the leaves of are decocted and used by Chinese Mongolians, Koreans, and Tibetans to treat skin itching, dry cracks, and other skin ailments, which indicates leaves may have a potential inhibitory effect on various skin inflammations. To examine the protective effect against skin inflammations of leaf extract (ATLE), an dermatitis model was established using sodium dodecyl sulfate (SLS)-induced HaCaT cells. The anti-inflammatory effect of ATLE was evaluated by analyzing cell viability, apoptosis, reactive oxygen species (ROS), interleukin 6 (IL-6), and prostaglandin E2 (PGE2) levels. Orthogonal experiments showed that the pretreatment with ATLE can reduce the IL-6 levels, PGE2 levels, and apoptosis increased in SLS-stimulated HaCaT cells, which indicates that ATLE has positive efficacy for dermatitis. Furthermore, three flavonoid compounds kaempferol-3---L-rhamnoside, quercetin-3---L-rhamnopyranoside, kaempferol-3,7-di---L-rhamnoside, and 1,2,3,4,6-Penta--galloyl--D-glucopyranose (PGG) were isolated and identified. Among them, kaempferol-3,7-di---L-rhamnoside was isolated from this plant for the first time. These compounds have been proven to have an anti-inflammatory effect. They may contribute to the efficacy of treating skin inflammation. The results revealed that ATLE has the potential to be used as an additive in various skin care products to prevent skin inflammations and may be incorporated in formulations for topical application as a therapeutic approach against dermatitis.
地锦槭（无患子科，以前归为槭树科）是一种原产于中国的高大落叶乔木。传统上，中国蒙古族、朝鲜族和藏族会将地锦槭的叶子煎水用于治疗皮肤瘙痒、干裂和其他皮肤疾病，这表明地锦槭叶可能对各种皮肤炎症具有潜在的抑制作用。为了研究地锦槭叶提取物（ATLE）对皮肤炎症的保护作用，使用十二烷基硫酸钠（SLS）诱导HaCaT细胞建立了接触性皮炎模型。通过分析细胞活力、凋亡、活性氧（ROS）、白细胞介素6（IL-6）和前列腺素E2（PGE2）水平来评估ATLE的抗炎作用。正交实验表明，用ATLE预处理可以降低SLS刺激的HaCaT细胞中升高的IL-6水平、PGE2水平和凋亡，这表明ATLE对接触性皮炎具有积极疗效。此外，还分离并鉴定了三种黄酮类化合物山柰酚-3-O-L-鼠李糖苷、槲皮素-3-O-L-鼠李吡喃糖苷、山柰酚-3,7-二-O-L-鼠李糖苷和1,2,3,4,6-五-O-没食子酰基-β-D-吡喃葡萄糖（PGG）。其中，山柰酚-3,7-二-O-L-鼠李糖苷是首次从该植物中分离得到。这些化合物已被证明具有抗炎作用。它们可能有助于地锦槭治疗皮肤炎症的功效。结果表明，ATLE有潜力用作各种护肤品中的添加剂以预防皮肤炎症，并且可以作为治疗接触性皮炎的一种治疗方法纳入局部应用制剂中。
