Han Chunhao, Chen Yifan, Shi Lei, Chen Hui, Li Lanhui, Ning Zhonghua, Zeng Dan, Wang Dehe
College of Animal Science and Technology, Hebei Agricultural University, Baoding, China.
College of Animal Science and Technology, China Agricultural University, Beijing, China.
Front Vet Sci. 2023 Mar 16;10:1116126. doi: 10.3389/fvets.2023.1116126. eCollection 2023.
Eggshell membranes (ESM) contain 90% protein, 3% lipids, 2% sugars, and small amounts of minerals such as calcium and magnesium. Of the 90% of proteins present, 472 proteins species have been identified. ESM provide the initial mineralization platform for eggshell formation, and can be used for to produce adsorbents, cosmetics, and medical products because of their special physical structure and chemical composition. The special physical structure of the eggshell membrane, with disulfide bonds between and within the protein molecules and the cross-linking of lysine-derived and heterochain chains between the eggshell membrane, makes the membrane very difficult to dissolve, with a maximum solubility rate of only 62%. Also, the insolubility of ESM limits its development and use also any related research. Based on the physical structure and chemical composition of the eggshell membrane, this paper reviews the latest research on eggshell membrane separation and membrane protein solubilization to provide a reference for promoting the separation, dissolution, and rational development and use of the avian eggshell membrane.
蛋壳膜（ESM）含有90%的蛋白质、3%的脂质、2%的糖类以及少量矿物质，如钙和镁。在其所含的90%的蛋白质中，已鉴定出472种蛋白质。蛋壳膜为蛋壳形成提供了初始矿化平台，由于其特殊的物理结构和化学成分，可用于生产吸附剂、化妆品和医疗产品。蛋壳膜的特殊物理结构，蛋白质分子之间及内部存在二硫键，且蛋壳膜之间赖氨酸衍生链和杂链交联，使得该膜极难溶解，最大溶解度仅为62%。此外，蛋壳膜的不溶性也限制了其开发利用以及任何相关研究。基于蛋壳膜的物理结构和化学成分，本文综述了蛋壳膜分离和膜蛋白增溶的最新研究，为促进禽蛋壳膜的分离、溶解以及合理开发利用提供参考。
