Irgang Rute, Poblete-Morales Matías, Avendaño-Herrera Ruben
Universidad Andrés Bello, Laboratorio de Patología de Organismos Acuáticos y Biotecnología Acuícola, Facultad de Ciencias de la Vida, Viña del Mar, Chile.
Interdisciplinary Center for Aquaculture Research (INCAR), Viña del Mar, Chile.
Int J Syst Evol Microbiol. 2023 Apr;73(4). doi: 10.1099/ijsem.0.005815.
Strain I-SCBP12n, a novel Gram-stain-negative, aerobic, non-spore-forming, motile-by-gliding and rod-shaped bacterium, was isolated from a chinstrap penguin chick () during a 2015 expedition to the Chilean Antarctic territory. Phylogenetic analysis based on 16S rRNA gene sequencing confirmed that strain I-SCBP12n belonged to the genus , being closely related to strains P3160 (98.52 %), WB 4.2-33 (98.47 %) and LM-19-Fp (98.47 %). The genome size of strain I-SCBP12n was 3.69 Mb with DNA G+C content 31.95 mol%. Genomic comparisons of strain I-SCBP12n with type species in the genus were performed, with obtained average values near 75.17 and 84.33 % for the blast and MUMer analyses of average nucleotide identity, respectively, and 0.86 for the tetranucleotides frequency analysis. These values are far from the accepted species cut-off values. Strain I-SCBP12n contained MK-6 as the predominant menaquinone and the major polar lipids were aminophospholipid, an unidentified aminolipid and unidentified lipids. The predominant fatty acids (> 5 %) were iso-C, iso-C, anteiso-C, iso-C, iso-C, iso-C 3-OH, C 6 and summed feature 3 (C 7c/C 6). Phenotypic, chemotaxonomic and genomic data supported the assignment of strain I-SCBP12n (=CECT 30404=RGM 3223) to a novel species of , for which the name sp. nov.is proposed.
菌株I-SCBP12n是一种新型革兰氏阴性、需氧、无芽孢、滑行运动的杆状细菌，于2015年对智利南极领地的一次考察中，从一只南极帽带企鹅幼崽中分离得到。基于16S rRNA基因测序的系统发育分析证实，菌株I-SCBP12n属于该属，与菌株P3160（98.52%）、WB 4.2-33（98.47%）和LM-19-Fp（98.47%）密切相关。菌株I-SCBP12n的基因组大小为3.69 Mb，DNA G+C含量为31.95 mol%。对菌株I-SCBP12n与该属模式种进行了基因组比较，在平均核苷酸同一性的blast分析和MUMer分析中，分别获得了接近75.17%和84.33%的平均值，四核苷酸频率分析的值为0.86。这些值远低于公认的物种截止值。菌株I-SCBP12n以MK-6作为主要甲基萘醌，主要极性脂类为氨基磷脂、一种未鉴定的氨基脂类和未鉴定的脂类。主要脂肪酸（>5%）为异-C、异-C、anteiso-C、异-C、异-C、异-C 3-OH、C 6和总和特征3（C 7c/C 6）。表型、化学分类和基因组数据支持将菌株I-SCBP12n（=CECT 30404=RGM 3223）归为该属的一个新物种，为此提出新物种名为sp. nov.。
