The Key Laboratory of Geriatrics, Beijing Institute of Geriatrics, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology of National Health Commission, Beijing, P.R. China.
National Center for Clinical Laboratories, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology, Beijing, P.R. China.
Int J Food Sci Nutr. 2023 Mar;74(2):234-246. doi: 10.1080/09637486.2023.2187742. Epub 2023 Apr 4.
Trimethylamine N-oxide (TMAO), a gut microbiota-dependent metabolite, has been shown to aggravate cardiovascular disease. However, the mechanisms of TMAO in the setting of cardiovascular disease progress remain unclear. Here, we aim to investigate the effects of TMAO on atherosclerosis (AS) development and the underlying mechanisms. Apoe -/- mice received choline or TMAO supplementation in a normal diet and a western diet for 12 weeks. Choline or TMAO supplementation in both normal diet and western diet significantly promoted plaque progression in Apoe-/- mice. Besides, serum lipids levels and inflammation response in the aortic root were enhanced by choline or TMAO supplementation. In particular, choline or TMAO supplementation in the western diet changed intestinal microbiota composition and bile acid metabolism. Therefore, choline or TMAO supplementation may promote AS by modulating gut microbiota in mice fed with a western diet and by other mechanisms in mice given a normal diet, even choline or TMAO supplementation in a normal diet can promote AS.
三甲基胺 N-氧化物（TMAO）是一种依赖于肠道微生物群的代谢物，已被证明会加重心血管疾病。然而，TMAO 在心血管疾病进展中的作用机制仍不清楚。在这里，我们旨在研究 TMAO 对动脉粥样硬化（AS）发展的影响及其潜在机制。Apoe -/- 小鼠在正常饮食和西方饮食中接受胆碱或 TMAO 补充 12 周。在正常饮食和西方饮食中补充胆碱或 TMAO 均可显著促进 Apoe-/- 小鼠斑块的进展。此外，血清脂质水平和主动脉根部的炎症反应也因胆碱或 TMAO 的补充而增强。特别是，在西方饮食中补充胆碱或 TMAO 会改变肠道微生物群组成和胆汁酸代谢。因此，胆碱或 TMAO 补充可能通过调节西方饮食喂养的小鼠肠道微生物群，以及通过在正常饮食中给予小鼠的其他机制来促进 AS，即使在正常饮食中补充胆碱或 TMAO 也可以促进 AS。
