Department of Molecular Biophysics & Biochemistry, Yale University, New Haven, Connecticut, USA; email:
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut, USA; email:
Annu Rev Biochem. 2023 Jun 20;92:175-198. doi: 10.1146/annurev-biochem-052521-035949. Epub 2023 Apr 5.
Chemical modifications on mRNA represent a critical layer of gene expression regulation. Research in this area has continued to accelerate over the last decade, as more modifications are being characterized with increasing depth and breadth. mRNA modifications have been demonstrated to influence nearly every step from the early phases of transcript synthesis in the nucleus through to their decay in the cytoplasm, but in many cases, the molecular mechanisms involved in these processes remain mysterious. Here, we highlight recent work that has elucidated the roles of mRNA modifications throughout the mRNA life cycle, describe gaps in our understanding and remaining open questions, and offer some forward-looking perspective on future directions in the field.
mRNA 的化学修饰代表了基因表达调控的一个关键层面。在过去的十年中，随着更多修饰被以越来越深和广的程度进行特征描述，该领域的研究一直在加速。已经证明，mRNA 修饰会影响从核转录合成的早期阶段到细胞质中降解的几乎每一个步骤，但在许多情况下，这些过程中涉及的分子机制仍然是神秘的。在这里，我们重点介绍了最近阐明 mRNA 修饰在整个 mRNA 生命周期中的作用的工作，描述了我们理解中的差距和遗留的问题，并对该领域的未来方向提供了一些前瞻性的观点。
