College of Pharmaceutical Science & Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Hebei University, Baoding 071002, China.
College of Pharmaceutical Science & Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, Hebei University, Baoding 071002, China.
Carbohydr Polym. 2023 Jul 1;311:120757. doi: 10.1016/j.carbpol.2023.120757. Epub 2023 Mar 2.
In this paper, the mixed solution of sodium alginate (SA) and arginine (Arg) was dried into a film and then crosslinked with zinc ion to form sodium alginate-arginine-zinc ion (SA-Arg-Zn) hydrogel for skin wound dressings. SA-Arg-Zn hydrogel had higher swelling ability, which was beneficial to absorbing wound exudate. Moreover, it exhibited antioxidant activity and strong inhibition against E. coli and S. aureus, and had no obvious cytotoxicity to NIH 3T3 fibroblasts. Compared with other dressings utilized in rat skin wound, SA-Arg-Zn hydrogel showed better wound healing efficacy and the wound closure ratio reached to 100 % on the 14th day. The result of Elisa test indicated that SA-Arg-Zn hydrogel down-regulated the expression of inflammatory factors (TNF-α and IL-6) and promoted the growth factor levels (VEGF and TGF-β). Furthermore, H&E staining results confirmed that SA-Arg-Zn hydrogel could reduce wound inflammation and accelerate re-epithelialization, angiogenesis and wound healing. Therefore, SA-Arg-Zn hydrogel is an effective and innovative wound dressing, moreover, the preparation technique is simple and feasible for industrial application.
本文将海藻酸钠（SA）和精氨酸（Arg）的混合溶液干燥成膜，然后用锌离子交联形成海藻酸钠-精氨酸-锌离子（SA-Arg-Zn）水凝胶，用于皮肤伤口敷料。SA-Arg-Zn 水凝胶具有更高的溶胀能力，有利于吸收伤口渗出物。此外，它具有抗氧化活性和对大肠杆菌和金黄色葡萄球菌的强抑制作用，对 NIH 3T3 成纤维细胞无明显细胞毒性。与大鼠皮肤伤口中使用的其他敷料相比，SA-Arg-Zn 水凝胶显示出更好的伤口愈合效果，第 14 天伤口闭合率达到 100%。Elisa 试验结果表明，SA-Arg-Zn 水凝胶下调了炎症因子（TNF-α和 IL-6）的表达，并促进了生长因子水平（VEGF 和 TGF-β）。此外，H&E 染色结果证实，SA-Arg-Zn 水凝胶可以减少伤口炎症，加速上皮化、血管生成和伤口愈合。因此，SA-Arg-Zn 水凝胶是一种有效且创新的伤口敷料，而且其制备技术简单可行，适用于工业应用。
