Ivanova E A
Ontogenez. 1977;8(2):150-8.
The dynamics of testosterone-7alpha-3H (T-3H) uptake by the developing genital system organs, as well as by the suprarenals, brain cortex and muscles was studied in vivo experiments with the 14-20 days old female rat embryos. Besides, the T3H uptake by the reproductive tract tissues and muscles just after the isolation of embryos from the uterus and after 2 days cultivation in the diffusion chambers implanted in the abdominal cavity of the adult castrated rats was studied in in vitro experiments. Moreover, the T3H uptake by the embryonic tissues was determined in the absence of ovaries. The specific uptake was noted in all the tissues at all developmental stages under all experimental conditions. The selective T3H uptake was noted during the periods corresponding to the rudiments' morphogenesis carachteristic for males. In the absence of ovaries, the T3H uptake by the reproductive tract tissues in vitro experiments decreased, but after 2 days cultivation in the diffusion chambers increased. The possible participation of ovaries in the female sex differentiation and the bisexual nature of rudiments determined by the presence of receptors both to estro- and androgens are discussed.
在对14 - 20日龄雌性大鼠胚胎进行的体内实验中，研究了发育中的生殖系统器官以及肾上腺、大脑皮层和肌肉对睾酮 - 7α - 3H（T - 3H）的摄取动态。此外，在体外实验中，研究了将胚胎从子宫分离后以及在植入成年去势大鼠腹腔的扩散室中培养2天后，生殖道组织和肌肉对T3H的摄取情况。此外，还在没有卵巢的情况下测定了胚胎组织对T3H的摄取。在所有实验条件下，所有组织在所有发育阶段均观察到特异性摄取。在与雄性雏形形态发生特征相对应的时期观察到选择性T3H摄取。在没有卵巢的情况下，体外实验中生殖道组织对T3H的摄取减少，但在扩散室中培养2天后增加。讨论了卵巢在雌性性别分化中的可能作用以及由雌激素和雄激素受体的存在所决定的雏形的双性性质。
