Department of Physical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. listopadu 12, 771 46, Olomouc, Czech Republic.
Regional Centre of Advanced Technologies and Materials, Czech Advanced Technology and Research Institute, Palacký University in Olomouc, Křížkovského 511/8, 779 00, Olomouc, Czech Republic.
Sci Rep. 2022 Mar 25;12(1):5222. doi: 10.1038/s41598-022-09294-7.
The number of antibiotic-resistant bacterial strains is increasing due to the excessive and inappropriate use of antibiotics, which are therefore becoming ineffective. Here, we report an effective way of enhancing and restoring the antibacterial activity of inactive antibiotics by applying them together with a cyanographene/Ag nanohybrid, a nanomaterial that is applied for the first time for restoring the antibacterial activity of antibiotics. The cyanographene/Ag nanohybrid was synthesized by chemical reduction of a precursor material in which silver cations are coordinated on a cyanographene sheet. The antibacterial efficiency of the combined treatment was evaluated by determining fractional inhibitory concentrations (FIC) for antibiotics with different modes of action (gentamicin, ceftazidime, ciprofloxacin, and colistin) against the strains Escherichia coli, Pseudomonas aeruginosa, and Enterobacter kobei with different resistance mechanisms. Synergistic and partial synergistic effects against multiresistant strains were demonstrated for all of these antibiotics except ciprofloxacin, which exhibited an additive effect. The lowest average FICs equal to 0.29 and 0.39 were obtained for colistin against E. kobei and for gentamicin against E. coli, respectively. More importantly, we have experimentally confirmed for the first time, that interaction between the antibiotic's mode of action and the mechanism of bacterial resistance strongly influenced the combined treatment's efficacy.
由于抗生素的过度和不适当使用，导致抗生素耐药菌株的数量不断增加，因此抗生素的疗效正在下降。在这里，我们报告了一种通过将抗生素与一种新型纳米材料——氰基石墨烯/银纳米杂化材料联合应用来增强和恢复抗生素抗菌活性的有效方法。该纳米杂化材料是通过在氰基石墨烯片上配位银离子的前体制备，并通过化学还原法合成的。通过测定不同作用模式（庆大霉素、头孢他啶、环丙沙星和黏菌素）的抗生素对具有不同耐药机制的大肠杆菌、铜绿假单胞菌和产酸克雷伯菌的菌株的部分抑菌浓度（FIC），评估了联合治疗的抗菌效率。除了环丙沙星表现出相加作用外，所有这些抗生素对多耐药菌株均表现出协同和部分协同作用。对于黏菌素对产酸克雷伯菌和庆大霉素对大肠杆菌的作用，获得了最低的平均 FIC 值，分别为 0.29 和 0.39。更重要的是，我们首次通过实验证实，抗生素作用模式与细菌耐药机制之间的相互作用强烈影响联合治疗的效果。
