Centro di Senologia della Svizzera Italiana (CSSI), Ente Ospedaliero Cantonale (EOC), Lugano, Switzerland.
Centro di Competenza Senologica, Gruppo Ospedaliero Moncucco, Lugano, Switzerland.
Ann Biomed Eng. 2023 Jul;51(7):1535-1546. doi: 10.1007/s10439-023-03159-4. Epub 2023 Apr 15.
This paper describes a cancer detection procedure based on customised electrical impedance spectroscopy (EIS) in breast cancer surgical samples and an analysis of its outcomes. A tissue analyser was developed to inject a low-amplitude alternating current with penetrating electrodes into breast specimens along a broad spectrum of frequencies. Experimental measurements were carried out on more than one hundred excised breast cancer specimens, with the goal of discriminating between the tumour and surrounding non-neoplastic tissue. The probe was inserted in different locations immediately after surgical excision in order to measure tissue impedance (modulus and phase). Electrical impedance varied significantly between neoplastic and surrounding non-neoplastic tissues, with a low standard deviation among the different measurements, confirming good reproducibility. Tumours could be discriminated from non-neoplastic tissues according to their impedance modulus value for high frequencies and phase value for low frequencies. Impedance also varied significantly in both non-neoplastic and tumour tissues depending on the patient's age and tumour characteristics, such as size and histological sub-type. EIS is able to discriminate between healthy tissue and cancer. Future developments of this technology could be exploited for intraoperative real-time evaluation of the transition zone between cancer and normal tissues.
本文描述了一种基于定制化电化学阻抗谱（EIS）的癌症检测方法，用于乳腺癌手术样本，并分析了其结果。我们开发了一种组织分析仪，通过穿透电极在乳房标本中注入低振幅交流电，并在广泛的频率范围内进行分析。我们对一百多个切除的乳腺癌标本进行了实验测量，旨在区分肿瘤和周围非肿瘤组织。探针在手术切除后立即插入不同位置，以测量组织阻抗（模和相）。肿瘤和周围非肿瘤组织的阻抗差异显著，不同测量之间的标准偏差较低，证实了良好的可重复性。根据高频时的阻抗模值和低频时的相位值，可以区分肿瘤和非肿瘤组织。阻抗在非肿瘤和肿瘤组织中也根据患者的年龄和肿瘤特征（如大小和组织学亚型）而显著变化。EIS 能够区分健康组织和癌症。这项技术的未来发展可以用于术中实时评估癌症和正常组织之间的过渡区域。
