Jiang Housen, Xu Qin, Wang Xiaolin, Shi Lin, Yang Xuedong, Sun Jianmin, Mei Xifan
Dalian Medical University, Dalian 116044, Liaoning, China.
Department of Hand and Foot Orthopedic Surgery, Weifang People's Hospital, Weifang 261042, Shandong, China.
ACS Omega. 2023 Mar 30;8(14):12653-12663. doi: 10.1021/acsomega.2c07266. eCollection 2023 Apr 11.
Diabetic foot ulcers with complex healing wounds accompanied by bacterial infection are considered a significant clinical problem which are made worse by the lack of effective treatments. Traditional antibiotics and dressings have failed to address wound infection and healing, and multifunctional combination therapies are attractive for treating chronic wounds. In this study, arginine (Arg) was loaded onto the surface of silver nanoclusters and encapsulated in a hydrogel to achieve antibacterial, anti-inflammatory, angiogenic, and collagen deposition functions through the slow release of Arg combined with silver nanoclusters. In vitro studies indicated that Arg-Ag@H composites inhibited methicillin-resistant Staphylococcus aureus and by 94 and 97%, respectively. The inhibition of bacterial biofilms reached 85%, and the migration ability of human venous endothelial cells (HUVECs) increased by 50%. In vitro studies showed that Arg-Ag@H composites increased the healing area of wounds by 26% and resulted in a 98% skin wound-healing rate. Safety studies confirmed the excellent biocompatibility of Arg-Ag@H. The results suggest that Arg-Ag@H offers new possibilities for treating chronic diabetic wounds.
伴有细菌感染的复杂愈合伤口的糖尿病足溃疡被认为是一个重大的临床问题，而缺乏有效的治疗方法使情况更加恶化。传统的抗生素和敷料未能解决伤口感染和愈合问题，多功能联合疗法对于治疗慢性伤口具有吸引力。在本研究中，精氨酸（Arg）被负载到银纳米簇表面并封装在水凝胶中，通过Arg与银纳米簇的缓慢释放实现抗菌、抗炎、血管生成和胶原蛋白沉积功能。体外研究表明，Arg-Ag@H复合材料对耐甲氧西林金黄色葡萄球菌的抑制率分别为94%和97%。对细菌生物膜的抑制率达到85%，人静脉内皮细胞（HUVECs）的迁移能力提高了50%。体外研究表明，Arg-Ag@H复合材料使伤口愈合面积增加了26%，皮肤伤口愈合率达到98%。安全性研究证实了Arg-Ag@H具有优异的生物相容性。结果表明，Arg-Ag@H为治疗慢性糖尿病伤口提供了新的可能性。
