Kong Changchang, Chen Shijunyin, Ge Wenhao, Zhao Yang, Xu Xiaodong, Wang Shiming, Zhang Jianfa
Center for Molecular Metabolism, Nanjing University of Science & Technology, Nanjing, Jiangsu, 210094, People's Republic of China.
Int J Nanomedicine. 2022 Jun 13;17:2629-2641. doi: 10.2147/IJN.S366899. eCollection 2022.
In order to overcome the inflammatory response to bacterial infection during wound healing, we have fabricated an antibacterial and anti-inflammatory wound dressing based on polysaccharide riclin and silver nanoparticles (AgNPs).
The riclin-AgNPs nanocomposite was developed by borohydride method and was characterized by UV-Vis, TEM, XRD, Zeta potential, DLS. In vitro, we assessed the cumulative release, antibacterial activities and cytotoxicity. In vivo, we examined the wound healing in mice wound infection experiment and inflammatory mediators using histological observations and gene expression analysis.
The riclin/AgNPs nanocomposite hydrogel exhibited nanosized orbicular particles with high purity and stability. In vitro, the riclin/AgNPs showed sustained release of AgNPs, effective suppression in pathogen growth and negligible toxicity toward mammalian fibroblasts and macrophage cells. In vivo, the riclin/AgNPs treatment leads to faster and smoother growth of fresh skin with suppressed expression of inflammatory mediators.
The reported Riclin-AgNPs nanocomposite hydrogel showed both antibacterial and anti-inflammatory functions, which induce significantly accelerated wound healing, indicating great potential as a novel attractive wound dressing material.
为了克服伤口愈合过程中对细菌感染的炎症反应，我们制备了一种基于多糖瑞克林和银纳米颗粒（AgNPs）的抗菌抗炎伤口敷料。
通过硼氢化物法制备瑞克林-AgNPs纳米复合材料，并通过紫外可见光谱、透射电子显微镜、X射线衍射、zeta电位、动态光散射进行表征。在体外，我们评估了其累积释放、抗菌活性和细胞毒性。在体内，我们通过组织学观察和基因表达分析，在小鼠伤口感染实验中检测伤口愈合情况和炎症介质。
瑞克林/AgNPs纳米复合水凝胶呈现出具有高纯度和稳定性的纳米级圆形颗粒。在体外，瑞克林/AgNPs显示出AgNPs的持续释放，对病原体生长有有效抑制作用，对哺乳动物成纤维细胞和巨噬细胞的毒性可忽略不计。在体内，瑞克林/AgNPs处理导致新鲜皮肤生长更快、更平滑，炎症介质的表达受到抑制。
所报道的瑞克林-AgNPs纳米复合水凝胶具有抗菌和抗炎功能，可显著加速伤口愈合，表明其作为一种新型有吸引力的伤口敷料材料具有巨大潜力。
