University General Hospital of Patras, Department of Microbiology, Patras, Greece.
University General Hospital of Patras, Department of Dermatology, Patras, Greece.
Rev Inst Med Trop Sao Paulo. 2023 Apr 14;65:e29. doi: 10.1590/S1678-9946202365029. eCollection 2023.
Acid-fast bacteria can be implicated in skin and soft tissue infections. Diagnostic identification can be challenging or not feasible by routine laboratory techniques, especially if there is no access to the Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) technology. Here, we present two cases of skin and soft tissue infections caused by two different acid-fast bacteria, Nocardia brasiliensis and Mycobacterium marinum. They both grew on Löwenstein-Jensen medium, Sabouraud agar medium and blood agar medium. Both bacteria appeared acid-fast by Ziehl-Neelsen stain and Gram-positive by Gram stain. The identification was performed by MALDI-TOF MS and gene analysis. N. brasiliensis and nontuberculous mycobacterium M. marinum represent rare pathogens that cause severe skin and soft tissue infections. Failure to identify the causative agent and subsequent inappropriate or inadequate treatment may lead to severe complications or even disseminated disease, especially in immunocompromised individuals.
可从皮肤和软组织感染中分离出抗酸杆菌。如果无法使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）技术，则通过常规实验室技术进行诊断鉴定可能具有挑战性或不可行。在此，我们介绍两例由两种不同的抗酸杆菌（巴西诺卡氏菌和海分枝杆菌）引起的皮肤和软组织感染。它们都在 Löwenstein-Jensen 培养基、沙保弱琼脂培养基和血琼脂培养基上生长。两种细菌均经齐-尼染色法显示抗酸性，革兰氏染色法显示革兰氏阳性。通过 MALDI-TOF MS 和基因分析进行鉴定。巴西诺卡氏菌和非结核分枝杆菌海分枝杆菌是引起严重皮肤和软组织感染的罕见病原体。未能识别病原体以及随后的不适当或不充分治疗可能导致严重并发症，甚至播散性疾病，尤其是在免疫功能低下的个体中。
