Fang Qi, Lai Yong, Zhang Dan, Lei Hui, Wang Fang, Guo Xiurong, Song Can
School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, China.
World J Microbiol Biotechnol. 2023 Apr 20;39(7):167. doi: 10.1007/s11274-023-03616-1.
Oudemansiella raphanipes is a type of fungus used as both medicine and food. Fungal polysaccharides have demonstrated various bioactivities, involving the adjust and control of gut microbiota, but no such studies on O. raphanipes polysaccharides (OrPs) have been reported. It is by extracting and purifying that OrPs was obtained from O. raphanipes crude polysaccharide and study their effects in mice. The sample contents of total sugar was 97.26%, and the monosaccharide content comprised mannose, rhamnose, glucose, and xylose in a molar ratio of 35.2:2.8:21.2:40.8. The effects of OrPs on body weight (BW), gut microbiota, fecal short-chain fatty acids (SCFAs), and the correlation between fecal SCFAs and gut microbes, in mice were investigated. The results of the experiment found that OrPs significantly (P < 0.01) inhibited the increase in BW, altered the constitution of the gut microbiota, and significantly (P < 0.05) enhanced the content of fecal SCFAs in mice. Moreover, among the top ten bacteria in terms of relative abundance, the Lachnospiraceae and Lachnospiraceae NK4A136 groups were positively associated with the increased production of SCFAs. Other bacteria, such as Atopobiaceae and Bifidobacterium of Actinobacteriota, and Faecalibaculum, Dubosiella, and Clostridium sensu stricto 5 of Firmicutes, were also positively associated with higher content of fecal SCFAs. The results of the experiment suggest that OrPs have a potential prebiotic effect on gut microbiota and may prevent BW gain. Furthermore, the major producers of SCFAs were Firmicutes and Actinobacteriota.
长根奥德蘑是一种药食两用真菌。真菌多糖已显示出多种生物活性，包括对肠道微生物群的调节和控制，但尚未有关于长根奥德蘑多糖（OrPs）的此类研究报道。通过提取和纯化从长根奥德蘑粗多糖中获得了OrPs，并研究了它们对小鼠的影响。样品中总糖含量为97.26%，单糖含量包括甘露糖、鼠李糖、葡萄糖和木糖，摩尔比为35.2:2.8:21.2:40.8。研究了OrPs对小鼠体重（BW）、肠道微生物群、粪便短链脂肪酸（SCFAs）以及粪便SCFAs与肠道微生物之间相关性的影响。实验结果发现，OrPs显著（P < 0.01）抑制了BW的增加，改变了肠道微生物群的组成，并显著（P < 0.05）提高了小鼠粪便SCFAs的含量。此外，在相对丰度排名前十的细菌中，毛螺菌科和毛螺菌科NK4A136组与SCFAs产量增加呈正相关。其他细菌，如放线菌门的阿托波氏菌科和双歧杆菌属，以及厚壁菌门的粪杆菌属、杜氏菌属和严格梭菌属5，也与粪便SCFAs含量较高呈正相关。实验结果表明，OrPs对肠道微生物群具有潜在的益生元作用，可能预防体重增加。此外，SCFAs的主要产生菌是厚壁菌门和放线菌门。
