CSIRO Environment, Ecosciences Precinct, 41 Boggo Road, Dutton Park, QLD 4102, Australia.
CSIRO Environment, Ecosciences Precinct, 41 Boggo Road, Dutton Park, QLD 4102, Australia.
J Virol Methods. 2023 Jul;317:114732. doi: 10.1016/j.jviromet.2023.114732. Epub 2023 Apr 18.
The ongoing COVID-19 pandemic has emphasized the significance of wastewater surveillance in monitoring and tracking the spread of infectious diseases, including SARS-CoV-2. The wastewater surveillance approach detects genetic fragments from viruses in wastewater, which could provide an early warning of outbreaks in communities. In this study, we determined the concentrations of four types of endogenous viruses, including non-enveloped DNA (crAssphage and human adenovirus 40/41), non-enveloped RNA (enterovirus), and enveloped RNA (SARS-CoV-2) viruses, from wastewater samples using the adsorption-extraction (AE) method with electronegative HA membranes of different pore sizes (0.22, 0.45, and 0.80 µm). Our findings showed that the membrane with a pore size of 0.80 µm performed comparably to the membrane with a pore size of 0.45 µm for virus detection/quantitation (repeated measurement one-way ANOVA; p > 0.05). We also determined the recovery efficiencies of indigenous crAssphage and pepper mild mottle virus, which showed recovery efficiencies ranging from 50% to 94% and from 20% to 62%, respectively. Our results suggest that the use of larger pore size membranes may be beneficial for processing larger sample volumes, particularly for environmental waters containing low concentrations of viruses. This study offers valuable insights into the application of the AE method for virus recovery from wastewater, which is essential for monitoring and tracking infectious diseases in communities.
持续的 COVID-19 大流行强调了废水监测在监测和追踪传染病（包括 SARS-CoV-2）传播方面的重要性。废水监测方法可检测废水中病毒的遗传片段，从而为社区中的疫情爆发提供早期预警。在这项研究中，我们使用带负电的 HA 膜（孔径分别为 0.22、0.45 和 0.80µm）的吸附提取（AE）方法，从废水样本中确定了四种内源性病毒（无包膜 DNA（crAssphage 和人腺病毒 40/41）、无包膜 RNA（肠道病毒）和包膜 RNA（SARS-CoV-2））的浓度。我们的研究结果表明，对于病毒检测/定量，孔径为 0.80µm 的膜与孔径为 0.45µm 的膜性能相当（重复测量单向方差分析；p＞0.05）。我们还确定了本土 crAssphage 和辣椒轻斑驳病毒的回收率，回收率分别在 50%至 94%和 20%至 62%之间。我们的结果表明，使用较大孔径的膜可能有利于处理较大的样本量，特别是对于含有低浓度病毒的环境水样。本研究为从废水中回收病毒的 AE 方法的应用提供了有价值的见解，这对于监测和追踪社区中的传染病至关重要。
