Department of Dental Education, School of Dentistry, Chonnam National University, 77 Yongbong-ro, Buk-gu, Gwangju 61186, Korea.
Research Institute, Oradentics Inc., 1805-ho, 25 Seongsuil-ro-4-gil, Seongdong-gu, Seoul 04781, Korea.
Molecules. 2018 Aug 9;23(8):1984. doi: 10.3390/molecules23081984.
Recently, studies have explored the use of probiotics like the strain, CMU (oraCMU), for use as preventive dental medicine instead of chemical oral care methods. The present study was conducted to investigate the antibacterial properties of the cell-free supernatant (CFS) from this bacterium. Cell morphology using the scanning electron microscope, and the antibacterial effect of CFS under various growth conditions were evaluated. The production of hydrogen peroxide, organic acids, fatty acids, and secretory proteins was also studied. Most of the antibacterial effects of oraCMU against periodontal pathogens were found to be acid- and hydrogen peroxide-dose-dependent effects. Lactic acid, acetic acid, and citric acid were the most common organic acids. Among the 37 fatty acids, only 0.02% of oleic acid (C18:1n-9, ) was detected. Proteomic analysis of the oraCMU secretome identified a total of 19 secreted proteins, including -acetylmuramidase. This protein may be a potential anti-microbial agent effective against .
最近，研究探索了使用益生菌，如菌株 CMU（oraCMU），作为预防性牙科药物，而不是使用化学口腔护理方法。本研究旨在研究该细菌的无细胞上清液（CFS）的抗菌特性。使用扫描电子显微镜观察细胞形态，并评估 CFS 在各种生长条件下的抗菌效果。还研究了过氧化氢、有机酸、脂肪酸和分泌蛋白的产生。发现 oraCMU 对牙周病原体的大多数抗菌作用是酸和过氧化氢剂量依赖性的。乳酸、乙酸和柠檬酸是最常见的有机酸。在 37 种脂肪酸中，仅检测到 0.02%的油酸（C18:1n-9，）。oraCMU 分泌组的蛋白质组学分析共鉴定出 19 种分泌蛋白，包括 -乙酰胞壁质酶。这种蛋白质可能是一种潜在的抗微生物剂，对有效。
