Geisshirt Heidi A, Bonde Charlotte S, Marcussen Caroline, Mejer Helena, Williams Andrew R
Department of Veterinary and Animal Sciences, University of Copenhagen, DK-1870 Frederiksberg, Denmark.
Pathogens. 2023 Mar 29;12(4):536. doi: 10.3390/pathogens12040536.
Enteric helminth infection is an increasing concern in companion animals due to reports of resistance to commonly used anthelmintic drugs. Thus, the assessment of new therapeutic options such as bioactive dietary additives is of high importance. Here, we adapted egg hatch, larval migration, and larval motility assays to screen extracts of several natural ingredients against the canine hookworm , a prevalent parasite of dogs in northern Europe. Egg hatch and larval migration assays were established showing that the anthelmintic drugs levamisole and albendazole had strong anti-parasitic activity against , validating the use of these assays for the assessment of novel anti-parasitic substances. Subsequently, we identified that extracts from the seaweed , but not extracts from grape seed or chicory, significantly inhibited both hatching and larval migration. Finally, we showed that α-linolenic acid, a putative anti-parasitic compound from , also exhibited anti-parasitic activity. Collectively, our results established a platform for the screening for anthelmintic resistance or novel drug candidates against and highlighted the potential use of seaweed extracts as a functional food component to help control hookworm infection in dogs.
由于有报道称常用驱虫药出现耐药性，肠道蠕虫感染在伴侣动物中日益受到关注。因此，评估生物活性膳食添加剂等新的治疗选择至关重要。在此，我们调整了虫卵孵化、幼虫迁移和幼虫活力测定方法，以筛选几种天然成分的提取物对犬钩虫的作用，犬钩虫是北欧犬类中一种常见的寄生虫。建立了虫卵孵化和幼虫迁移测定方法，结果表明驱虫药左旋咪唑和阿苯达唑对犬钩虫具有很强的抗寄生虫活性，验证了这些测定方法可用于评估新型抗寄生虫物质。随后，我们发现海藻提取物而非葡萄籽或菊苣提取物能显著抑制虫卵孵化和幼虫迁移。最后，我们表明来自海藻的一种假定抗寄生虫化合物α-亚麻酸也具有抗寄生虫活性。总体而言，我们的研究结果建立了一个筛选抗驱虫药耐药性或针对犬钩虫的新型候选药物的平台，并突出了海藻提取物作为功能性食品成分在帮助控制犬类钩虫感染方面的潜在用途。
