Hearne A M, O'Sullivan M A, Atkins G J
J Gen Virol. 1986 Jun;67 ( Pt 6):1091-8. doi: 10.1099/0022-1317-67-6-1091.
Early mouse embryos at the four- to eight-cell stage or the blastocyst stage could be infected with the A7 strain of Semliki Forest virus (SFV) after the removal of the zona pellucida, either by Pronase treatment or following hatching of blastocysts. With SFV, rapid virus production and eventual cytolysis resulted from infection at either stage. For four- to eight-cell embryos the cytopathic effect was delayed and a proportion of embryos developed to the blastocyst stage. Four- and eight-cell embryos could not be infected with rubella virus (RV), even after removal of the zona pellucida. RV infection of zona-free blastocysts resulted in a productive but non-cytolytic infection which did not affect embryonic development to the early egg-cylinder stage. RV did not multiply in inner cell mass cells isolated from embryos at the blastocyst stage, although SFV did multiply in such cells.
在去除透明带后，处于4至8细胞期或囊胚期的早期小鼠胚胎，可通过链霉蛋白酶处理或囊胚孵化后，感染塞姆利基森林病毒（SFV）的A7毒株。对于这两个阶段的感染，SFV都会导致病毒快速产生并最终引起细胞溶解。对于4至8细胞期胚胎，细胞病变效应出现延迟，部分胚胎发育至囊胚期。即使去除透明带，风疹病毒（RV）也无法感染4至8细胞期胚胎。无透明带囊胚感染RV会产生一种有感染性但不引起细胞溶解的感染，这种感染不会影响胚胎发育至早期卵柱期。尽管SFV能在从囊胚期胚胎分离出的内细胞团细胞中增殖，但RV不能在这些细胞中增殖。
