在人类和牛的胎盘组织中感染时基因的表达。

Expression of Gene in the Placentas from Humans and Cows during Infection.

机构信息

Department of Microbiology, College of Veterinary Medicine, University of Basrah, Basrah, Iraq.

Department of Medicine, College of Veterinary Medicine, University of Basrah, Basrah, Iraq.

出版信息

Arch Razi Inst. 2022 Oct 31;77(5):1575-1586. doi: 10.22092/ARI.2022.358327.2192. eCollection 2022 Oct.

DOI:10.22092/ARI.2022.358327.2192

PMID:37123145

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10133627/

Abstract

Brucellosis is an important contagious disease affecting most domestic and mature animals. Since the impact of IL-1β in invasion and survival remains elusive, the current study sought to elucidate the actual roles of these potent cytokines in the modulation of the initial immune response to infection. Therefore, this study aimed to detect in the placenta of aborted women and cows and estimate the expression of the interleukin 1β () gene associated with immune response mechanisms to infection. The detection of was performed by Rose Bengal Test (RBT) and Polymerase Chain Reaction based gene (AlkB-PCR) in the sera and placenta samples of aborted women and cows, respectively. The overall percentage of infection was 13.1% and 5% as determined by RBT and -PCR in aborted women's sera and placentas, respectively. On the other hand, the overall percentage rates of infection in the sera and placentas from aborted cows were 30% and 11% as estimated by RBT and -PCR, respectively. The results of RBT demonstrated that the association between and abortion in cows was statistically significant. On the other hand, it was found that the association between and abortion in women was not significant. Moreover, according to the results of -based PCR, the association between and abortion was statistically significant in aborted cows, while it was not significant in aborted women. The sensitivity, specificity, and accuracy of RBT were calculated as 60.00, 53.85, and 54.55%, respectively. Moreover, positive and negative predictive values were reported as 14.33% and 91.28%, respectively. Regarding RBT for aborted cows, the sensitivity, specificity, and accuracy of the test were 81.82%, 57.78%, and 62.49%, respectively. The positive predictive value was reported as 32.08%, while the negative predictive value was reported as 92.88%. Quantitative PCR (qPCR) was carried out for the evaluation of Interleukin 1 Beta () gene expression. The qPCR result was presented as a fold change in gene expression. A significant increment of gene expression was observed in aborted women (114.905±99.661) and cows (22.454 ±18.528), compared to non-aborted women (4.953±5.564) and cows (2.033±1.845). Statistical comparison of gene expression between aborted women and cows illustrated a non-significant increment in gene expression in aborted women (114.905±99.661), compared to aborted cows (22.454 ±18.528).

摘要

布鲁氏菌病是一种重要的传染病，影响大多数国内和成熟动物。由于白细胞介素 1β（IL-1β）在入侵和存活中的作用仍不清楚，本研究旨在阐明这些强效细胞因子在调节对感染的初始免疫反应中的实际作用。因此，本研究旨在检测流产妇女和奶牛胎盘中的布鲁氏菌，并估计与感染免疫反应机制相关的白细胞介素 1β（IL-1β）基因的表达。通过 Rose Bengal 试验（RBT）和基于聚合酶链反应的基因（AlkB-PCR）分别在流产妇女和奶牛的血清和胎盘样本中检测。通过 RBT 和 -PCR 分别在流产妇女的血清和胎盘样本中确定的感染总百分比为 13.1%和 5%。另一方面，通过 RBT 和 -PCR 分别估计流产奶牛的血清和胎盘的感染总百分比为 30%和 11%。RBT 的结果表明，在奶牛中，与流产相关的与之间存在统计学显著关联。另一方面，发现与妇女流产相关的之间没有显著关联。此外，根据基于 -PCR 的结果，在流产奶牛中，与流产相关的之间存在统计学显著关联，而在流产妇女中则不显著。RBT 的灵敏度、特异性和准确性分别计算为 60.00、53.85 和 54.55%。此外，报告了阳性和阴性预测值分别为 14.33%和 91.28%。对于流产奶牛的 RBT，该测试的灵敏度、特异性和准确性分别为 81.82%、57.78%和 62.49%。阳性预测值为 32.08%，而阴性预测值为 92.88%。进行了定量 PCR（qPCR）以评估白细胞介素 1β（IL-1β）基因表达。qPCR 结果表示基因表达的倍数变化。与非流产妇女（4.953±5.564）和奶牛（2.033±1.845）相比，流产妇女（114.905±99.661）和奶牛（22.454 ±18.528）中观察到基因表达显著增加。流产妇女（114.905±99.661）与流产奶牛（22.454 ±18.528）之间基因表达的统计学比较表明，流产妇女中基因表达的增加无统计学意义（114.905±99.661）。