Physics and Technology Faculty, Kuban State University, 350040 Krasnodar, Russia.
Laboratory of Problems of Stable Isotope Spreading in Living Systems, Federal Research Center the Southern Scientific Center, Russian Academy of Sciences, 344006 Rostov-on-Don, Russia.
Int J Mol Sci. 2023 Apr 28;24(9):8046. doi: 10.3390/ijms24098046.
The crystal structure and the biological activity of a new coordination compound of magnesium ions with comenic acid, magnesium comenate, was characterized and studied. Quantitative and qualitative analysis of the compound was investigated in detail using elemental X-ray fluorescent analysis, thermal analysis, IR-Fourier spectrometry, UV spectroscopy, NMR spectroscopy, and X-ray diffraction analysis. Based on experimental analytical data, the empirical formula of magnesium comenate [Mg(HCom)(HO)]·2HO was established. This complex compound crystallizes with eight water molecules, six of which are the hydration shell of the Mg cation, and two more molecules bind the [Mg(HO)] aquacation with ionized ligand molecules by intermolecular hydrogen bonds. The packing of molecules in the crystal lattice is stabilized by a branched system of hydrogen bonds with the participation of solvate water molecules and oxygen atoms of various functional groups of ionized ligand molecules. With regard to the biological activity of magnesium comenate, a neuroprotective, stress-protective, and antioxidant effect was established in in vitro and in vivo models. In in vitro experiments, magnesium comenate protected cerebellar neurons from the toxic effects of glutamate and contributed to the preservation of neurite growth parameters under oxidative stress caused by hydrogen peroxide. In animal studies, magnesium comenate had a stress-protective and antioxidant effect in models of immobilization-cold stress. Oral administration of magnesium comenate at a dose of 2 mg/kg of animal body weight for 3 days before stress exposure and for 3 days during the stress period led to a decrease in oxidative damage and normalization of the antioxidant system of brain tissues against the background of induced stress. The obtained results indicate the advisability of further studies of magnesium comenate as a compound potentially applicable in medicine for the pharmacological correction of conditions associated with oxidative and excitotoxic damage to nerve cells.
研究了一种新型镁离子与冠醚酸配合物的晶体结构和生物活性。使用元素 X 射线荧光分析、热分析、红外傅里叶光谱、紫外光谱、核磁共振光谱和 X 射线衍射分析对该化合物进行了详细的定量和定性分析。基于实验分析数据，建立了镁冠醚酸[Mg（HCom）（HO）]·2HO 的经验式。该配合物结晶时有 8 个水分子，其中 6 个为 Mg 阳离子的水合壳，另外 2 个分子通过分子间氢键与带电荷的配体分子结合。晶格中分子的堆积由包含溶剂水分子和带电荷配体分子各官能团氧原子的支化氢键体系稳定。关于镁冠醚酸的生物活性，在体外和体内模型中建立了神经保护、应激保护和抗氧化作用。在体外实验中，镁冠醚酸保护小脑神经元免受谷氨酸的毒性作用，并有助于在过氧化氢引起的氧化应激下保持神经突生长参数。在动物研究中，镁冠醚酸在固定冷应激模型中具有应激保护和抗氧化作用。在应激暴露前 3 天和应激期间 3 天，每天以 2 毫克/千克动物体重的剂量口服给予镁冠醚酸，可降低氧化损伤并使脑组织抗氧化系统在诱导应激的背景下正常化。所得结果表明，进一步研究镁冠醚酸作为一种潜在可用于医学的化合物具有合理性，可用于药理学纠正与氧化应激和兴奋毒性损伤神经细胞相关的病症。
