State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Anal Chem. 2023 May 30;95(21):8376-8383. doi: 10.1021/acs.analchem.3c01123. Epub 2023 May 15.
A microfluidic gradient array is a widely used screening and analysis device, which has characteristics of high efficiency, high automation, and low consumption. Bipolar electrode electrochemiluminescence (BPE-ECL) has special value in microfluidic array chips. The combination of the microfluidic gradient and BPE arrays has potential for high-throughput screening. In this article, a microfluidic BPE array chip for gradient culture and conditional screening of cancer cells was designed. The generation of concentration gradients, continuous culture of cancer cells with high throughput, and drug screening through BPE-ECL of the Ru(bpy)/TPrA system can be performed in one chip. We tested gradient pro-oxidation of MCF-7 by ascorbic acid and the synergistic effect of pro-oxidation on doxorubicin. The method achieves high analysis efficiency through a BPE array while simplifying the tedious procedures required by cell culture methods.
微流控梯度阵列是一种广泛应用的筛选和分析装置，具有高效、自动化和低消耗的特点。双极电极电化学发光（BPE-ECL）在微流控阵列芯片中具有特殊价值。微流控梯度和 BPE 阵列的结合具有高通量筛选的潜力。本文设计了一种用于癌细胞梯度培养和条件筛选的微流控 BPE 阵列芯片。该芯片可在一个芯片中实现浓度梯度的产生、高通量的癌细胞连续培养以及通过 Ru(bpy)/TPrA 体系的 BPE-ECL 进行药物筛选。我们测试了 MCF-7 中抗坏血酸的促氧化作用和促氧化作用对阿霉素的协同作用。该方法通过 BPE 阵列实现了高分析效率，同时简化了细胞培养方法所需的繁琐步骤。
