Description and comparative genome analysis of Hallella absiana sp. nov., isolated from pig feces.

OBJECTIVE

The genus Hallella was described within Bacteroidaceae, and then reclassified within Prevotellaceae based on its phenotypic and phylogenetic description. It is associated with degradation of carbohydrate. However, some species of Hallella have pathobiotic properties, and are involved in infections and chronic inflammatory disorders.

METHODS

Here, we used a polyphasic taxonomic approach to characterize the two strains: YH-C38 and YH-C4B9b. A detailed metabolic analysis was conducted to compare the two novel isolates with related strains within the genus Hallella.

RESULT

Analysis of 16S rRNA gene sequences revealed that the isolates were most closely related to Hallella mizrahii JCM 34422 with 98.5% and 98.6% similarities, respectively. Analysis of the multi-locus species tree based on whole genome sequences of the isolates and related strains revealed that the isolates formed a sub-cluster adjacent to H. mizrahii JCM 34422. The average nucleotide identity values for YH-C38 and YH-C4B9b, and the most closely related strain H. mizrahii JCM 34422, were 93.5% and 93.8%, respectively. The main fatty acids were iso C 3OH and anteiso C. The predominant menaquinones were MK-12, MK-11, and MK-13. The cell wall contained the peptidoglycan of meso-diaminopimelic acid. Analysis of comparative metabolic analysis revealed that isolates YH-C38 and YH-C4B9b each contained 155 carbohydrate-active enzymes, and glycoside hydrolase was the largest family.

CONCLUSION

Two rod-shaped, obligately anaerobic, Gram-stain-negative bacteria, isolated from pig feces, were designated as strains YH-C38 and YH-C4B9b. Based on the chemotaxonomic, phenotypic, and phylogenetic properties, YH-C38 (=KCTC 25103 = JCM 35423) and YH-C4B9b (=KCTC 25104 = JCM 35609) represent a novel taxon. The name Hallella absiana sp. nov. is proposed.