ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
Vitabio, Inc., Daejeon, 305-500, Republic of Korea.
Anaerobe. 2023 Jun;81:102735. doi: 10.1016/j.anaerobe.2023.102735. Epub 2023 May 16.
The genus Hallella was described within Bacteroidaceae, and then reclassified within Prevotellaceae based on its phenotypic and phylogenetic description. It is associated with degradation of carbohydrate. However, some species of Hallella have pathobiotic properties, and are involved in infections and chronic inflammatory disorders.
Here, we used a polyphasic taxonomic approach to characterize the two strains: YH-C38 and YH-C4B9b. A detailed metabolic analysis was conducted to compare the two novel isolates with related strains within the genus Hallella.
Analysis of 16S rRNA gene sequences revealed that the isolates were most closely related to Hallella mizrahii JCM 34422 with 98.5% and 98.6% similarities, respectively. Analysis of the multi-locus species tree based on whole genome sequences of the isolates and related strains revealed that the isolates formed a sub-cluster adjacent to H. mizrahii JCM 34422. The average nucleotide identity values for YH-C38 and YH-C4B9b, and the most closely related strain H. mizrahii JCM 34422, were 93.5% and 93.8%, respectively. The main fatty acids were iso C 3OH and anteiso C. The predominant menaquinones were MK-12, MK-11, and MK-13. The cell wall contained the peptidoglycan of meso-diaminopimelic acid. Analysis of comparative metabolic analysis revealed that isolates YH-C38 and YH-C4B9b each contained 155 carbohydrate-active enzymes, and glycoside hydrolase was the largest family.
Two rod-shaped, obligately anaerobic, Gram-stain-negative bacteria, isolated from pig feces, were designated as strains YH-C38 and YH-C4B9b. Based on the chemotaxonomic, phenotypic, and phylogenetic properties, YH-C38 (=KCTC 25103 = JCM 35423) and YH-C4B9b (=KCTC 25104 = JCM 35609) represent a novel taxon. The name Hallella absiana sp. nov. is proposed.
Hallella 属最初被描述于拟杆菌科（Bacteroidaceae），随后基于表型和系统发育描述被重新归类于普雷沃氏菌科（Prevotellaceae）。它与碳水化合物的降解有关。然而，一些 Hallella 属的物种具有病理生物特性，并与感染和慢性炎症性疾病有关。
本研究采用多相分类方法对 YH-C38 和 YH-C4B9b 两株菌进行了表征。对两株新分离株与 Hallella 属内相关菌株进行了详细的代谢分析比较。
16S rRNA 基因序列分析表明，这两株菌与 Hallella mizrahii JCM 34422 的相似度最高，分别为 98.5%和 98.6%。基于两株菌及其相关菌株全基因组序列构建的多位点种系发生树分析表明，两株菌形成了一个与 H. mizrahii JCM 34422 相邻的亚群。YH-C38 和 YH-C4B9b 与最密切相关的菌株 H. mizrahii JCM 34422 的平均核苷酸同一性值分别为 93.5%和 93.8%。主要脂肪酸为 iso C 3OH 和 anteiso C。主要的menaquinone 为 MK-12、MK-11 和 MK-13。细胞壁含有 meso-二氨基庚二酸的肽聚糖。比较代谢分析结果表明，两株菌 YH-C38 和 YH-C4B9b 各含有 155 种碳水化合物活性酶，其中糖苷水解酶是最大的家族。
从猪粪便中分离得到两株杆状、严格厌氧、革兰氏阴性细菌，被命名为 YH-C38 和 YH-C4B9b 菌株。基于化学分类、表型和系统发育特征，YH-C38（=KCTC 25103=JCM 35423）和 YH-C4B9b（=KCTC 25104=JCM 35609）代表一个新的分类群。提议将其命名为 Hallella absiana sp. nov.。
