State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, Nanjing 210023, China.
Department of Dermatology, Department of Clinical Laboratory Medicine, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing 210008, China.
ACS Appl Mater Interfaces. 2023 May 31;15(21):25427-25436. doi: 10.1021/acsami.3c03630. Epub 2023 May 19.
The treatment of cutaneous wounds involving complex biological processes has become a significant public health concern worldwide. Here, we developed an efficient extracellular vesicle (EV) ink to regulate the inflammatory microenvironment and promote vascular regeneration for wound healing. The technology, termed portable bioactive ink for tissue healing (PAINT), leverages bioactive M2 macrophage-derived EVs (EV) and a sodium alginate precursor, forming a biocompatible EV-Gel within 3 min after mixing, enabling it to be smeared on wounds in situ to meet diverse morphologies. The bioactive EV reprogram macrophage polarization and promote the proliferation and migration of endothelial cells, thereby effectively regulating inflammation and enhancing angiogenesis in wounds. Through integration with a 3D printing pen, the platform enables EV-Gel to be applied to wound sites having arbitrary shapes and sizes with geometric matches for tissue repairment. When evaluated using a mouse wound model, PAINT technology accelerates cutaneous wound healing by promoting the angiogenesis of endothelial cells and the polarization of macrophages to M2 phenotype in vivo, demonstrating the high potential of bioactive EV ink as a portable biomedical platform for healthcare.
涉及复杂生物过程的皮肤伤口治疗已成为全球范围内的一个重大公共卫生关注点。在这里，我们开发了一种高效的细胞外囊泡（EV）墨水，以调节炎症微环境并促进血管再生，从而实现伤口愈合。该技术被称为用于组织愈合的便携式生物活性墨水（PAINT），利用生物活性 M2 巨噬细胞衍生的 EV（EV）和海藻酸钠前体，在混合后 3 分钟内形成生物相容性的 EV-Gel，使其能够涂抹在原位伤口上，以满足各种形态的需要。生物活性 EV 可重新编程巨噬细胞极化，并促进内皮细胞的增殖和迁移，从而有效调节炎症并增强伤口中的血管生成。通过与 3D 打印笔集成，该平台可将 EV-Gel 应用于具有任意形状和大小的伤口部位，并与组织修复实现几何匹配。在使用小鼠伤口模型进行评估时，PAINT 技术通过促进内皮细胞的血管生成和巨噬细胞向 M2 表型的极化来加速皮肤伤口愈合，证明了生物活性 EV 墨水作为便携式生物医学平台用于医疗保健的巨大潜力。
