Whelan W L, Gocke E, Manney T R
Genetics. 1979 Jan;91(1):35-51. doi: 10.1093/genetics/91.1.35.
A system of strains and growth media was developed to allow efficient detection of forward mutation, reversion, complementation, and suppression at the canavanine-resistance (CAN1) locus of Saccharomyces cerevisiae. Genetic fine-structure analysis revealed that the map length is at least 40, and possibly as much as 60 X-ray map units; this is the longest gene map yet reported in S. cerevisiae. Allelic complementation was not observed, despite testing of a large number of allele pairs, and alleles suppressible by the ochre suppressor SUP11 were absent from a sample of 48 spontaneous mutants and occurred infrequently (7%) among a sample of ultraviolet-induced mutants. Infrequent mutant types included canavanine-resistant mutants capable of arginine uptake and alleles thought to represent deletions or inversions. In contrast to previous reports in the literature, the spontaneous forward mutation rate at CAN1 did not increase during meiosis.
构建了一个菌株和生长培养基系统，以实现对酿酒酵母刀豆氨酸抗性（CAN1）位点的正向突变、回复突变、互补作用和抑制作用的高效检测。遗传精细结构分析表明，图谱长度至少为40，可能多达60个X射线图谱单位；这是酿酒酵母中迄今报道的最长基因图谱。尽管测试了大量等位基因对，但未观察到等位基因互补现象，并且在48个自发突变体样本中不存在可被赭石型抑制基因SUP11抑制的等位基因，在紫外线诱导突变体样本中也很少出现（7%）。罕见的突变类型包括能够摄取精氨酸的刀豆氨酸抗性突变体以及被认为代表缺失或倒位的等位基因。与文献中之前的报道相反，减数分裂期间CAN1位点的自发正向突变率并未增加。
