Department of Mechanical & Aerospace Engineering, University of Florida, Gainesville, Florida, United States of America.
J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida, United States of America.
PLoS One. 2023 May 19;18(5):e0286000. doi: 10.1371/journal.pone.0286000. eCollection 2023.
Immune cells and stromal cells regulate wound healing and regeneration through complex activation patterns with spatiotemporal variation. The scarless regeneration of Spiny mice (Acomys species) is no exception; differential activation of immune and stromal cell populations seems to play a role in its remarkable regenerative capacity. To elucidate the role and interplay of Acomys immune cells in mammalian regeneration, we sought to create Acomys-Mus chimeras by transplanting bone marrow (BM) from Acomys into NOD Scid Gamma (NSG), a severely immunodeficient mouse line often used in creating humanized mice. Here, we report that Acomys BM cells fail to reconstitute and differentiate when transferred to irradiated NSG adults and neonates. In addition, we did not detect the presence of donor cells nor observe the onset of Graft versus Host Disease (GvHD)-like pathology, even after transplanting Acomys splenocytes in Acomys-Mus chimeras suggesting early graft failure. Overall, these results demonstrate the adoptive transfer of Acomys BM alone is not sufficient to establish Acomys hematopoietic system in NSG mouse.
免疫细胞和基质细胞通过时空变化的复杂激活模式调节伤口愈合和再生。无疤痕再生的沙鼠（Acomys 物种）也不例外；免疫细胞和基质细胞群体的差异激活似乎在其显著的再生能力中发挥作用。为了阐明 Acomys 免疫细胞在哺乳动物再生中的作用和相互作用，我们试图通过将 Acomys 的骨髓 (BM) 移植到严重免疫缺陷的 NOD Scid Gamma (NSG) 小鼠中，来创建 Acomys-Mus 嵌合体，这种小鼠通常用于创建人源化小鼠。在这里，我们报告说，当将 Acomys BM 细胞转移到辐射处理的 NSG 成体和新生儿时，它们无法重建和分化。此外，即使在 Acomys-Mus 嵌合体中移植 Acomys 脾细胞后，我们也没有检测到供体细胞的存在，也没有观察到移植物抗宿主病 (GvHD)-样病理学的发生，这表明早期移植物失功。总的来说，这些结果表明，单独的 Acomys BM 过继转移不足以在 NSG 小鼠中建立 Acomys 造血系统。
