Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Science, Xinjiang Normal University, Urumqi, China.
Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, China.
BMC Genomics. 2023 May 19;24(1):273. doi: 10.1186/s12864-023-09313-5.
Previous studies have shown that secondary metabolites of Bacillus subtilis strain Z15 (BS-Z15) are effective in treating fungal infections in mice. To evaluate whether it also modulates immune function in mice to exert antifungal effects, we investigated the effect of BS-Z15 secondary metabolites on both the innate and adaptive immune functions of mice, and explored its molecular mechanism through blood transcriptome analysis.
The study showed that BS-Z15 secondary metabolites increased the number of monocytes and platelets in the blood, improved natural killer (NK) cell activity and phagocytosis of monocytes-macrophages, increased the conversion rate of lymphocytes in the spleen, the number of T lymphocytes and the antibody production capacity of mice, and increased the levels of Interferon gamma (IFN-γ), Interleukin-6 (IL-6), Immunoglobulin G (IgG) and Immunoglobulin M (IgM) in plasma. The blood transcriptome analysis revealed 608 differentially expressed genes following treatment with BS-Z15 secondary metabolites, all of which were significantly enriched in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) terms for immune-related entries and pathways such as Tumor Necrosis Factor (TNF) and Toll-like receptor (TLR) signaling pathways, and upregulated expression levels of immune-related genes such as Complement 1q B chain (C1qb), Complement 4B (C4b), Tetracyclin Resistant (TCR) and Regulatory Factor X, 5 (RFX5).
BS-Z15 secondary metabolites were shown to enhance innate and adaptive immune function in mice, laying a theoretical foundation for its development and application in the field of immunity.
先前的研究表明，枯草芽孢杆菌 Z15 菌株（BS-Z15）的次生代谢产物对治疗小鼠真菌感染有效。为了评估其是否通过调节免疫功能发挥抗真菌作用，我们研究了 BS-Z15 次生代谢产物对小鼠固有和适应性免疫功能的影响，并通过血液转录组分析探讨了其分子机制。
研究表明，BS-Z15 次生代谢产物增加了血液中单核细胞和血小板的数量，提高了自然杀伤（NK）细胞的活性和单核细胞-巨噬细胞的吞噬作用，增加了脾淋巴细胞的转化率、T 淋巴细胞的数量和小鼠的抗体产生能力，并提高了血浆中干扰素γ（IFN-γ）、白细胞介素 6（IL-6）、免疫球蛋白 G（IgG）和免疫球蛋白 M（IgM）的水平。血液转录组分析显示，BS-Z15 次生代谢产物处理后有 608 个差异表达基因，这些基因均显著富集于与免疫相关的基因本体论（GO）和京都基因与基因组百科全书（KEGG）条目，如肿瘤坏死因子（TNF）和 Toll 样受体（TLR）信号通路，并上调了免疫相关基因如补体 1q B 链（C1qb）、补体 4B（C4b）、T 细胞受体（TCR）和调节因子 X、5（RFX5）的表达水平。
BS-Z15 次生代谢产物增强了小鼠的固有和适应性免疫功能，为其在免疫领域的开发和应用奠定了理论基础。
