Markandey Manasvini, Bajaj Aditya, Verma Mahak, Virmani Shubi, Singh Mukesh Kumar, Gaur Preksha, Das Prasenjit, Srikanth C V, Makharia Govind, Kedia Saurabh, Ahuja Vineet
Department of Gastroenterology, All India Institute of Medical Sciences, New Delhi, India.
Regional Centre for Biotechnology, 3rd Milestone Gurugram-Faridabad Expressway, Faridabad 121001, India.
iScience. 2023 Apr 23;26(5):106738. doi: 10.1016/j.isci.2023.106738. eCollection 2023 May 19.
A crypt autochthonous microbial population called crypt-associated microbiota (CAM) is localized intimately with gut regenerative and immune machinery. The present report utilizes laser capture microdissection coupled with 16S amplicon sequencing to characterize the CAM in patients with ulcerative colitis (UC) before and after fecal microbiota transplantation with anti-inflammatory diet (FMT-AID). Compositional differences in CAM and its interactions with mucosa-associated microbiota (MAM) were compared between the non-IBD controls and in patients with UC pre- and post-FMT (n = 26). Distinct from the MAM, CAM is dominated by aerobic members of Actinobacteria and Proteobacteria and exhibits resilience of diversity. CAM underwent UC-associated dysbiosis and demonstrated restoration post-FMT-AID. These FMT-restored CAM taxa correlated negatively with disease activity in patients with UC. The positive effects of FMT-AID extended further in refurbishing CAM-MAM interactions, which were obliterated in UC. These results encourage investigation into host-microbiome interactions established by CAM, to understand their role in disease pathophysiology.
一种称为隐窝相关微生物群（CAM）的隐窝原生微生物群体与肠道再生和免疫机制紧密相关。本报告利用激光捕获显微切割技术结合16S扩增子测序，对接受抗炎饮食粪便微生物群移植（FMT-AID）前后的溃疡性结肠炎（UC）患者的CAM进行表征。比较了非IBD对照组与UC患者FMT前后（n = 26）CAM的组成差异及其与黏膜相关微生物群（MAM）的相互作用。与MAM不同，CAM以放线菌和变形菌的需氧成员为主，且表现出多样性的恢复力。CAM经历了与UC相关的生态失调，并在FMT-AID后表现出恢复。这些FMT恢复的CAM分类群与UC患者的疾病活动呈负相关。FMT-AID在修复CAM-MAM相互作用方面的积极作用进一步扩展，而这种相互作用在UC中被破坏。这些结果鼓励对由CAM建立的宿主-微生物组相互作用进行研究，以了解它们在疾病病理生理学中的作用。
