Department of Nephrology, Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine, Wenzhou, 325000, China.
Department of Nephrology, Wenzhou Central Hospital, Wenzhou, 325000, China.
Adv Rheumatol. 2023 May 22;63(1):24. doi: 10.1186/s42358-023-00306-2.
The relationship between humidity and systemic lupus erythematosus (SLE) has yielded inconsistent results in prior research, while the effects of humidity on lupus in animal experiments and its underlying mechanism remain inadequately explored.
The present study aimed to investigate the impact of high humidity (80 ± 5%) on lupus using female and male MRL/lpr mice, with a particular focus on elucidating the role of gut microbiota in this process. To this end, fecal microbiota transplantation (FMT) was employed to transfer the gut microbiota of MRL/lpr mice under high humidity to blank MRL/lpr mice under normal humidity (50 ± 5%), allowing for an assessment of the effect of FMT on lupus.
The study revealed that high humidity exacerbated lupus indices (serum anti-dsDNA, ANA, IL-6, and IFN- g, and renal pathology) in female MRL/lpr mice but had no significant effect on male MRL/lpr mice. The aggravation of lupus caused by high humidity may be attributed to the increased abundances of the Rikenella, Romboutsia, Turicibacter, and Escherichia-Shigella genera in female MRL/lpr mice. Furthermore, FMT also exacerbated lupus in female MRL/lpr mice but not in male MRL/lpr mice.
In summary, this study has demonstrated that high humidity exacerbated lupus by modulating gut microbiota in female MRL/lpr mice. The findings underscore the importance of considering environmental factors and gut microbiota in the development and progression of lupus, particularly among female patients.
先前的研究表明，湿度与系统性红斑狼疮（SLE）之间的关系存在不一致的结果，而湿度对动物实验中狼疮的影响及其潜在机制仍未得到充分探索。
本研究旨在通过雌性和雄性 MRL/lpr 小鼠研究高湿度（80±5%）对狼疮的影响，特别关注肠道微生物群在这一过程中的作用。为此，采用粪便微生物群移植（FMT）将高湿度下 MRL/lpr 小鼠的肠道微生物群移植到正常湿度（50±5%）下的空白 MRL/lpr 小鼠中，评估 FMT 对狼疮的影响。
研究表明，高湿度加剧了雌性 MRL/lpr 小鼠的狼疮指数（血清抗 dsDNA、ANA、IL-6 和 IFN-γ以及肾脏病理学），但对雄性 MRL/lpr 小鼠没有显著影响。高湿度引起的狼疮加重可能归因于雌性 MRL/lpr 小鼠中 Rikenella、Romboutsia、Turicibacter 和 Escherichia-Shigella 属的丰度增加。此外，FMT 也加剧了雌性 MRL/lpr 小鼠的狼疮，但对雄性 MRL/lpr 小鼠没有影响。
综上所述，本研究表明，高湿度通过调节雌性 MRL/lpr 小鼠的肠道微生物群加重狼疮。这些发现强调了在狼疮的发生和进展中考虑环境因素和肠道微生物群的重要性，尤其是在女性患者中。
